usage
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Quality Level
shelf life
1 yr at 2‑8 °C
packaging
pkg of 1 kit
application(s)
cell analysis
foreign activity
nuclease and protease, free
shipped in
wet ice
storage temp.
2-8°C
General description
Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。
Application
适于作为细胞核成分的来源，为体外凋亡检测和功能研究提供细胞核成分。
Biochem/physiol Actions
配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核，包括贴壁 （例如，HEK293和COS7）和非贴壁（例如，Jurkat和HFN7.1）细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
Other Notes
所有操作步骤应在冰块上或2-8°C条件下进行。
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
相关内容
Instructions
Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NUC101-1KT | 04061834211662 |