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MP0035

Sigma-Aldrich

LookOut ® 支原体 PCR 检测试剂盒

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

别名:

PCR-based mycoplasma screening

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

质量水平

100

包装

pkg of 1 kit

技术

PCR: suitable

应用

detection
microbiology

相容性

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

储存温度

2-8°C

一般描述

该LookOut®支原体PCR检测试剂盒利用聚合酶链反应（PCR），这是因其在对细胞培养基中的支原体、无胆甾原体和尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法，整体的检测时间得到了大幅度的下降。

应用

支原体PCR检测试剂盒视频实验方法
LookOut®支原体PCR检测试剂盒已用于支原体感染筛查。

相容性

该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3′-5′外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。

法律信息

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
BCCL4114
BCCL4119
BCCL8332
BCCK5837
BCCJ8830

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  3. How many tests can be performed with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    The kit contains 8 positive control reaction tubes and 24 test reaction tubes.  A positive control and negative control should be run with each assay.  Depending upon the number of samples run with each assay, the kit could test up to 24 samples.

  4. Which species of Mycoplasma will Product MP0035, LookOut® Mycoplasma PCR Detection Kit, recognize?

    Detection of all mycoplasma species tested so far and usually encountered as contaminants in cell cultures  This kit will detect A. laidlawii, M. agalactiae, M. arginini , M. arthritidis, M. bovis, M. cloacale, M. falconis, M. faucium, M. fermentans, M. hominis, M. hyorhinis, M. hyosynoviae, M. opalescens, M. orale, M. primatum, M. pulmonis, M. salivarium, M. spermatophilum, M. timone.  This kit is not suitable for detection of M. pneumoniae, U. urealyticum, or other clinically associated species.

  5. How much DNA is necessary for use with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    Detection of Mycoplasma sp. requires less than 5 mycoplasma genomes per microliter of sample.  This is approximately 1-5 fg of DNA.

  6. When using Product MP0035, LookOut® Mycoplasma PCR Detection Kit, there is no band for the internal control?

    When a customer observes a band with the positive control and no band for the negative control, it is possible that the Taq could be the problem. We observe a lack of sensitivity with some polymerases from other manufacturers. The activity of the polymerases was enough to amplify the positive control but not the internal amplification control. The internal amplification control is the meter for the sensitivity of the PCR and the activity of the Taq used. The positive control contains more DNA and is good for result evaluation, only. We recommend that the customer use Jump Start Taq (Product No. D9307) as indicated in the protocol.

  7. Product MP0035, LookOut® Mycoplasma PCR Detection Kit does not contain TAQ.  What DNA polymerase should be used?

    The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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