跳转至内容
Merck
CN

MP0025

Venor™ GeM 支原体检测试剂盒，基于 PCR

别名:

PCR mycoplasma detection kit

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

usage

 kit sufficient for 25 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

Quality Level

200

相关类别

Application

基于 PCR 的 Venor™ GeM 支原体检测试剂盒采用 PCR 技术快速可靠地检测细胞培养物和病毒原液中的支原体 DNA。

General description

Venor™ GeM 支原体检测试剂盒利用聚合酶链反应（PCR），成为检测细胞培养物和其它细胞培养衍生生物制品中支原体和无胆甾原体污染的灵敏度最高的首选方法。检测需要低至 1-5fg 的支原体 DNA，对应于每个样品 2-5 个支原体。引物组对高度保守的 rRNA 操纵子有特异性，更具体而言，对支原体基因组中的 16S rRNA 编码区有特异性。这让我们可以检测迄今为止测试的所有支原体、无胆甾原体和脲原体物种，这些物种通常是细胞培养物中的污染物。该试剂盒不可扩增真核和细菌 DNA。

Other Notes

不包括Taq聚合酶。 优化用于 D9307、Taq DNA 聚合酶

Preparation Note

该试剂盒在环境温度下运输时是稳定的。收货后，在 2–8 °C下储存。 引物/核苷酸混合物、阳性对照和内对照再水化后，在 –20 °C 以下储存，避免反复冻融。对于低样品数的重复测试，引物/核苷酸混合物和对照应在再水化后等分。遵循这些建议，该试剂盒在标签上注明的失效日期之前是稳定的。

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000483162
0000483162
0000406903
0000406903
0000353055

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Product MP0025, VenorGeM® Mycoplasma Kit, does not contain TAQ.  What  DNA polymerase should be used?

    Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that can be used include Taq, Tfl, and Tth. The kit has been optimized for use with Product No. D9307.

  2. When using Product MP0025, VenorGeM® Mycoplasma Kit, there is inhibition in the sample (no internal control band).  Can the sample be diluted and the assay repeated?

    Sigma Aldrich does not recommend diluting the sample and re-assay as this can result in false negatives (sample diluted below the detection limits of the assay).  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  3. Can a cell lysate be used in the VenorGeM® Mycoplasma Kit, Product MP0025, assay?

    A cell lysate can contain PCR inhibitors.  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  4. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  5. The sample required for VenorGeM® Mycoplasma Kit, Product MP0025, is a cell culture supernatant.  Is this from adherent or suspension cells?

    The mycoplasma assay kit can be run on supernatants from either adherent or suspension cells. The centrifugation step during sample preparation will remove any cellular debris to limit PCR inhibitors.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yanyan Li et al.
Oncotarget, 8(63), 106311-106323 (2018-01-02)
Decades of cytotoxic and more recently immunotherapy treatments for malignant glioma have had limited success due to dynamic intra-tumoral heterogeneity. The dynamic interplay of cancer cell subpopulations has been found to be under the control of proteins in the cancer
Anbarasu Kannan et al.
Scientific reports, 9(1), 11632-11632 (2019-08-14)
Triple negative breast cancer (TNBC) has poor survival, exhibits rapid metastases, lacks targeted therapies and reliable prognostic markers. Here, we examined metastasis promoting role of cancer testis antigen SPANXB1 in TNBC and its utility as a therapeutic target and prognostic
Enhanced neonatal Fc receptor function improves protection against primate SHIV infection.
Ko SY, Pegu A, Rudicell RS, et al.
Nature, 514(7524), 642-645 (2014)
Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
查看所有相关文献

商品

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

查看所有结果

实验方案

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持