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安全信息

MP0025

Sigma-Aldrich

Venor™ GeM 支原体检测试剂盒，基于 PCR

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别名:
PCR mycoplasma detection kit
UNSPSC代码:
12352207
NACRES:
NA.75

用途

 kit sufficient for 25 tests

质量水平

200

包装

pkg of 1 kit

储存条件

dry at room temperature

技术

PCR: suitable

应用

detection
microbiology

储存温度

2-8°C

相关类别

一般描述

Venor™ GeM 支原体检测试剂盒利用聚合酶链反应（PCR），成为检测细胞培养物和其它细胞培养衍生生物制品中支原体和无胆甾原体污染的灵敏度最高的首选方法。检测需要低至 1-5fg 的支原体 DNA，对应于每个样品 2-5 个支原体。引物组对高度保守的 rRNA 操纵子有特异性，更具体而言，对支原体基因组中的 16S rRNA 编码区有特异性。这让我们可以检测迄今为止测试的所有支原体、无胆甾原体和脲原体物种，这些物种通常是细胞培养物中的污染物。该试剂盒不可扩增真核和细菌 DNA。

应用

基于 PCR 的 Venor™ GeM 支原体检测试剂盒采用 PCR 技术快速可靠地检测细胞培养物和病毒原液中的支原体 DNA。

组分

不包括Taq聚合酶。 优化用于 D9307、Taq DNA 聚合酶

储存及稳定性

该试剂盒在环境温度下运输时是稳定的。收货后，在 2–8 °C下储存。 引物/核苷酸混合物、阳性对照和内对照再水化后，在 –20 °C 以下储存，避免反复冻融。对于低样品数的重复测试，引物/核苷酸混合物和对照应在再水化后等分。遵循这些建议，该试剂盒在标签上注明的失效日期之前是稳定的。

法律信息

Venor is a trademark of Minerva Biolabs GmbH

仅试剂盒组分

产品编号
说明
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

WGK

WGK 3

法规信息

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分析证书(COA)

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  1. Product MP0025, VenorGeM® Mycoplasma Kit, does not contain TAQ.  What  DNA polymerase should be used?

    Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that can be used include Taq, Tfl, and Tth. The kit has been optimized for use with Product No. D9307.

  2. When using Product MP0025, VenorGeM® Mycoplasma Kit, there is inhibition in the sample (no internal control band).  Can the sample be diluted and the assay repeated?

    Sigma Aldrich does not recommend diluting the sample and re-assay as this can result in false negatives (sample diluted below the detection limits of the assay).  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  3. Can a cell lysate be used in the VenorGeM® Mycoplasma Kit, Product MP0025, assay?

    A cell lysate can contain PCR inhibitors.  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  4. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  5. The sample required for VenorGeM® Mycoplasma Kit, Product MP0025, is a cell culture supernatant.  Is this from adherent or suspension cells?

    The mycoplasma assay kit can be run on supernatants from either adherent or suspension cells. The centrifugation step during sample preparation will remove any cellular debris to limit PCR inhibitors.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Evolution of Hoxa11 regulation in vertebrates is linked to the pentadactyl state
Kherdjemil Y, et al.
Nature (2016)
Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Anthony J Bainor et al.
Cancer research, 77(19), 5339-5348 (2017-08-16)
Distinguishing between indolent and aggressive prostate adenocarcinoma remains a priority to accurately identify patients who need therapeutic intervention. SIN3B has been implicated in the initiation of senescence
Enhanced neonatal Fc receptor function improves protection against primate SHIV infection.
Ko SY, Pegu A, Rudicell RS, et al.
Nature, 514(7524), 642-645 (2014)
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