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MP0035

Sigma-Aldrich

LookOut® Mycoplasma PCR Detection Kit

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

Synonym(s):

PCR-based mycoplasma screening

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

quality

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

Quality Level

100

packaging

pkg of 1 kit

technique(s)

PCR: suitable

application(s)

detection
microbiology

compatibility

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

storage temp.

2-8°C

General description

The LookOut® Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA in concentrations of 20,000 to 20 copies.
The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. Total assay time is greatly reduced compared to general protocols that require individual loading of reaction tubes.

Application

Mycoplasma PCR Detection Kit Video Protocol
The LookOut® Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.

Compatibility

The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3′-5′ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

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Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  3. How many tests can be performed with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    The kit contains 8 positive control reaction tubes and 24 test reaction tubes.  A positive control and negative control should be run with each assay.  Depending upon the number of samples run with each assay, the kit could test up to 24 samples.

  4. Which species of Mycoplasma will Product MP0035, LookOut® Mycoplasma PCR Detection Kit, recognize?

    Detection of all mycoplasma species tested so far and usually encountered as contaminants in cell cultures  This kit will detect A. laidlawii, M. agalactiae, M. arginini , M. arthritidis, M. bovis, M. cloacale, M. falconis, M. faucium, M. fermentans, M. hominis, M. hyorhinis, M. hyosynoviae, M. opalescens, M. orale, M. primatum, M. pulmonis, M. salivarium, M. spermatophilum, M. timone.  This kit is not suitable for detection of M. pneumoniae, U. urealyticum, or other clinically associated species.

  5. How much DNA is necessary for use with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    Detection of Mycoplasma sp. requires less than 5 mycoplasma genomes per microliter of sample.  This is approximately 1-5 fg of DNA.

  6. When using Product MP0035, LookOut® Mycoplasma PCR Detection Kit, there is no band for the internal control?

    When a customer observes a band with the positive control and no band for the negative control, it is possible that the Taq could be the problem. We observe a lack of sensitivity with some polymerases from other manufacturers. The activity of the polymerases was enough to amplify the positive control but not the internal amplification control. The internal amplification control is the meter for the sensitivity of the PCR and the activity of the Taq used. The positive control contains more DNA and is good for result evaluation, only. We recommend that the customer use Jump Start Taq (Product No. D9307) as indicated in the protocol.

  7. Product MP0035, LookOut® Mycoplasma PCR Detection Kit does not contain TAQ.  What DNA polymerase should be used?

    The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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