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Key Documents

Safety Information

11663925910

Roche

Mycoplasma PCR ELISA

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25 MG
CN¥1,401.70

CN¥1,401.70


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25 MG
CN¥1,401.70

About This Item

UNSPSC Code:
12352200

CN¥1,401.70


Please contact Customer Service for Availability
New, lower price on this item!

usage

sufficient for 96 reactions

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

Specificity

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Application

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.[1][2]
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.[3][4]

Features and Benefits

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Packaging

1 kit containing 14 components.

Sequence

Chain Length Positive control: 520 bp.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Lysis Reagent

  • Neutralization Reagent

  • PCR Mix ready-to-use

  • Denaturation Reagent

  • Hybridization Buffer

  • Biotinylated Capture Probe

  • Washing Buffer 10x concentrated

  • Anti-Digoxigenin-POD antibody

  • Conjugate Dilution Buffer

  • TMB Substrate Solution

  • Stop Reagent

  • Positive Control DNA

  • Streptavidin-coated Microplate

  • Adhesive Plate Cover Foils

See All (14)

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Regulatory Information

监管及禁止进口产品

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    Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi
    Margaryan N V, et al.
    International Journal of Molecular Sciences, 17(3), 418-418 (2016)
    Pengfei Wang et al.
    Nature, 593(7857), 130-135 (2021-03-09)
    The COVID-19 pandemic has had widespread effects across the globe, and its causative agent, SARS-CoV-2, continues to spread. Effective interventions need to be developed to end this pandemic. Single and combination therapies with monoclonal antibodies have received emergency use authorization1-3
    Novel Non-phosphorylated Serine 9/21 GSK3?/a Antibodies: Expanding the Tools for Studying GSK3 Regulation
    Tessa G, et al.
    Frontiers in Molecular Neuroscience (2016)
    Novel Non-phosphorylated Serine 9/21 GSK3?/? Antibodies: Expanding the Tools for Studying GSK3 Regulation
    Grabinski T, et al.
    Frontiers in Molecular Neuroscience, 9, 123-123 (2016)
    Tessa Grabinski et al.
    Frontiers in molecular neuroscience, 9, 123-123 (2016-12-03)
    Glycogen synthase kinase 3 (GSK3) β and α are serine/threonine kinases involved in many biological processes. A primary mechanism of GSK3 activity regulation is phosphorylation of N-terminal serine (S) residues (S9 in GSK3β, S21 in GSK3α). Phosphorylation is inhibitory to

    Protocols

    Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service