usage
sufficient for 96 reactions
manufacturer/tradename
Roche
shipped in
dry ice
storage temp.
−20°C
Application
支原体PCR ELISA已用于检测细胞系中的支原体污染。
仅供研究使用。不可用于诊断过程。
支原体PCR ELISA试剂盒已被用于检测细胞培养物或其他样品材料中支原体/无胆甾原体/脲原体的污染。
支原体PCR ELISA试剂盒已被用于检测细胞培养物或其他样品材料中支原体/无胆甾原体/脲原体的污染。
Biochem/physiol Actions
支原体PCR ELISA可用于检测以下所有支原体、无胆甾原体、和脲原体物种：M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum.未检测到来自其他细菌（Clostridium sp.除外）、酵母和真核细胞的DNA。
Features and Benefits
检测时间： 大约6个小时
样品材料：细胞培养上清液、细胞悬浮液或其他样品
灵敏度：每毫升培养基至少1×103个菌落形成单位（cfu）的支原体
样品材料：细胞培养上清液、细胞悬浮液或其他样品
灵敏度：每毫升培养基至少1×103个菌落形成单位（cfu）的支原体
- 高灵敏度：可检测1-10fg DNA，相当于每个反应约1-20个基因拷贝（至少103cfu/ml）
- 检测范围广：到目前为止测试的所有支原体、无胆甾原体、和脲原体物种都可被检测到
- 简单：使用相同的实验方案检测所有的支原体，而不需要使用针对不同支原体物种不同培养条件的培养方法
- 快速的结果：整个过程可以在一天内完成
- 易于处理大量样品：即用型混合物和ELISA检测程序可实现快速和半自动样品处理
Other Notes
仅用于生命科学研究。不可用于诊断。
链长度阳性对照：520 bp。
Packaging
1个试剂盒包含14种组分。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
监管及禁止进口产品
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实验方案
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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