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Merck
CN

L7275

Sigma-Aldrich

LB肉汤(Lennox)

Tablet microbial growth medium

别名:

Luria Bertani肉汤

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

等级

for molecular biology

质量水平

无菌性

non-sterile

形式

tablet

技术

microbiological culture: suitable

应用

food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

一般描述

Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。 这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。
脱水Luria-Bertani (LB)培养基称为Lennox LB,以氨基酸作为碳源。

应用

使用LB培养基(Lennox)培养大肠肝菌和 and 细菌人工染色体(BAC)。
适用于 大肠杆菌 菌株的非选择性培养,用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养,包括需要低盐条件的抗生素,如吉欧霉素®

特点和优势

Lennox LB平板电脑提供:
  • 方便的小包装,无需称重
  • 快速准备–无需搅拌

组分

10 g/L胰蛋白胨
5克/升酵母提取物
5 g/L 氯化钠
2.2克/升惰性粘合剂

重悬

将每片1.1g片剂加入48.3毫升水中(无需搅拌)。在121℃高压灭菌20分钟。添加抗生素(如果需要)之前,请冷却。

法律信息

Zeocin is a registered trademark of Cayla Sarl

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference among the many LB - Lennox products?

    LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Synthesis of antibacterial surfaces by plasma grafting of zinc oxide based nanocomposites onto polypropylene
De Rancourt Y, et al.
Journal of Colloid and Interface Science, 402, 320-326 (2013)
Miller, J.H.
Experiments in Molecular Genetics null
Escherichia coli Physiology in Luria-Bertani Broth
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746-8746 (2007)
Simultaneous quantification of active carbon-and nitrogen-fixing communities and estimation of fixation rates using fluorescence in situ hybridization and flow cytometry
McInnes AS, et al.
Applied and Environmental Microbiology, 80(21), 6750-6759 (2014)
Fluorescence in situ hybridization of ductal lavage samples identifies malignant phenotypes from cytologically normal cells in women with breast cancer
Adduci KM, et al.
Cancer Cytopathology, 111(3), 185-191 (2007)

实验方案

General protocols for growth of competent cells in microbial medium.

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

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