跳转至内容
Merck
CN
所有图片(1)

文件

H8032

Sigma-Aldrich

Hanahan′s肉汤（SOB培养基）

powder microbial growth medium

别名:

超优肉汤培养基

登录查看公司和协议定价
所有图片(1)

About This Item

UNSPSC代码:
41106200
NACRES:
NA.85

等级

for molecular biology

质量水平

100

描述

recommended for cultivation of recombinant E. coli.

无菌性

non-sterile

形式

powder

组成

KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

技术

microbiological culture: suitable

pH值（酸碱度）

6.8-7.2(2.8% solution)

应用

food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

相关类别

一般描述

Hannahan’s 肉汤 (SOB)是用于制备和转化感受态细胞（大肠杆菌）的微生物生长培养基。 酪蛋白酶解产物和酵母提取物为重组 大肠杆菌提供含氮化合物和生长因子。氯化钾和氯化钠可维持细胞的等渗环境。培养基中加入硫酸镁，作为 DNA 复制的必要成分。

应用

建议将Hanahan′s Broth用于大肠杆菌的重组菌株的培养。它适用于制备感受态 大肠杆菌，通常添加0.4％的葡萄糖。它还可用于制备 钩虫贪铜菌 H16 感受态细胞。它适用于 大肠杆菌 菌株的非选择性培养，用于克隆、生产DNA质粒和生产重组蛋白。

特点和优势

Hannahan′s Broth（SOB）粉末提供：
  • 标准配方
  • 液体形式的廉价替代品

重悬

通过搅拌使28g粉末悬浮在1L水中。在121℃下高压灭菌15分钟。在加入添加物，例如抗生素（如果需要），之前请先冷却。

其他说明

酪蛋白酶酶解产物和酵母抽提物提供了必要的氮化合物和生长因子。 氯化钾和氯化钠为细胞提供了等渗环境。 硫酸镁有助于DNA复制。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Arenas-LC, et al.
Biotechnology for Biofuels, 12, 150-150 (2019)
Nicole Pearcy et al.
PLoS computational biology, 18(5), e1010106-e1010106 (2022-05-24)
Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis due to its ability to
Difco Manual, 444-444 (1998)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
查看所有相关文献

实验方案

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门