Application
建议将该产品以1-5 μg/cm2或0.5-50 μg/mL用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用和研究目标。
明胶已用于多种应用。 它可用于对细胞培养器皿进行包被以改善细胞的附着，加入至PCR以稳定Taq DNA，作为蛋白质印迹、ELISA和免疫化学中的封闭试剂，以及作为细菌学中用于物种分化培养基的组分。 作为一种生物相容性的聚合物，它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。在制药工业中，明胶可用作悬浮和包封剂，以及其他应用。
明胶已用于多种应用。 它可用于对细胞培养器皿进行包被以改善细胞的附着，加入至PCR以稳定Taq DNA，作为蛋白质印迹、ELISA和免疫化学中的封闭试剂，以及作为细菌学中用于物种分化培养基的组分。 作为一种生物相容性的聚合物，它已被作为递送载体用于活性生物分子的释放以及支架的生成用于组织工程应用。在制药工业中，明胶可用作悬浮和包封剂，以及其他应用。
Disclaimer
干燥的明胶，当在室温下储存于密闭容器中时，将可保持多年不变。 当在空气存在时在100℃下加热时，它会溶胀变软，并分解成碳质物质并伴有吡啶碱和氨的释放。
Other Notes
明胶存在于胶原蛋白中，是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。
Preparation Note
该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂，如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数，可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom指数变化于90至300 g之间。该产品具有175的凝胶强度。
存储类别
11 - Combustible Solids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
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