标签
FLAG® tagged
等级
for molecular biology
形式
buffered aqueous solution
运输
dry ice
储存温度
−20°C
一般描述
The pFLAG-CTC™ Expression Vector is a 5.3 kb E. coli expression vector used for cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein. The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products.
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb vE. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
C-terminal FLAG fusion proteins may be purified using Monoclonal ANTI-FLAG M2, Catalog Number F3165, and ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-CTS-BAP Control Plasmid is a 6.7 kb vE. coli plasmid used for efficient and controlled periplasmic expression of C-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
组分
- pFLAG-CTC™ Expression Vector 10 μg (E5394) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CTS™-BAP Control Plasmid 1 μg (P7707) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
原理
The promoter-regulatory region of the strong tac promoter (a hybrid of the trp and lac promoters from E.coli) drives transcription of ORF-FLAG fusion constructs. Control of transcription is regulated by the presence of the lacO sequences and inclusion of the lac repressor gene (lacI) on the plasmid.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CTC is a trademark of Sigma-Aldrich Co. LLC
pFLAG-CTS is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
法规信息
新产品
Ram P Garg et al.
Microbiology (Reading, England), 156(Pt 2), 472-483 (2009-11-07)
Streptomyces antibiotic regulatory proteins (SARPs) have been shown to activate transcription by binding to a tandemly arrayed set of heptameric direct repeats located around the -35 region of their cognate promoters. Experimental evidence is presented here showing that vlmI is
Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas.
Robin Couch et al.
BioTechniques, 32(6), 1230-1230 (2002-06-21)
Veronika Kucharova et al.
PloS one, 8(6), e66429-e66429 (2013-07-11)
mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability.
Rachel L Olsen et al.
Veterinary microbiology, 164(1-2), 164-170 (2013-02-26)
The human pathogens enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC), as well as the mouse pathogen Citrobacter rodentium encode type III secretion system (T3SS) effector proteins to promote their survival in the infected host. The mechanisms of action and
Tsuyoshi Miki et al.
Journal of bacteriology, 191(22), 6843-6854 (2009-09-22)
The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded
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Bacterial Expression Vectors: tac Promoter System
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