grade
Molecular Biology
Quality Level
form
lyophilized powder
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。
Application
Denhardt溶液已被用于：
- 作为杂交缓冲液的组分，用于脑切片的杂交
- 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
- 用于杂交混合物的制备
适合用于Southern和Northern印迹膜的封闭缓冲液。
Biochem/physiol Actions
Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。
Preparation Note
制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。
Still not finding the right product?
Explore all of our products under Denhardt′s溶液
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D9905-5X5ML | 04061833591345 |
| D9905-5ML | 04061833591338 |