推荐产品
等级
for molecular biology
质量水平
表单
lyophilized powder
技术
Southern blotting: suitable
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
2-8°C
一般描述
Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。
应用
Denhardt溶液已被用于：
- 作为杂交缓冲液的组分，用于脑切片的杂交
- 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
- 用于杂交混合物的制备
适合用于Southern和Northern印迹膜的封闭缓冲液。
生化/生理作用
Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。
组分
每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。
制备说明
制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
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