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Merck
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安全信息

D9905

Sigma-Aldrich

Denhardt′s溶液

for Northern and Southern blotting, lyophilized powder

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别名:
用于印迹技术的Denhardt′s 溶液
UNSPSC代码:
12161700
NACRES:
NA.25

等级

for molecular biology

质量水平

100

形式

lyophilized powder

技术

Southern blotting: suitable

异质活性

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

储存温度

2-8°C

相关类别

一般描述

Denhardt溶液是一种阻断剂，通常用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩液，可稀释至任何浓度，用于各种预杂交和杂交缓冲液。

应用

Denhardt溶液已被用于：
  • 作为杂交缓冲液的组分，用于脑切片的杂交
  • 用于原位杂交过氧化物酶-（抗过氧化物酶法-全标本包埋）（ISHPAP-WM）过程
  • 用于杂交混合物的制备

适合用于Southern和Northern印迹膜的封闭缓冲液。

生化/生理作用

Denhardt溶液和明胶-铬明矾可用于处理染色体，从而在原位杂交后用胰蛋白酶处理以实现染色体的g显带。

组分

每管Denhardt粉末含有50mg牛血清白蛋白（BSA）、50mg Ficoll和50mg聚乙烯吡咯烷酮（PVP）。

制备说明

制备50倍浓缩液，将全部内容物溶解在5 mL分子生物级水中。在-20°C下可储存长达两年。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

含少量动物源组分生物产品

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Molecular Cloning: A Laboratory Manual, 9-9 (1989)
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E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
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