D7291
脱氧核糖核酸酶 I 无 RNase 溶液 来源于牛胰腺
别名:
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
biological source
bovine pancreas
grade
Molecular Biology
form
buffered aqueous glycerol solution
specific activity
10000 units/mg protein
mol wt
29.1 kDa
concentration
5—15 mg protein/mL
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
foreign activity
RNase, none detected
storage temp.
−20°C
Quality Level
Gene Information
cow ... DNASE1(282217)
Application
在分子生物学应用中用于RNA纯化过程中去除DNA，用于制备DNA进行缺口翻译，以及通过足迹法分析DNA-蛋白质相互作用。
来自牛胰脏的无RNA酶脱氧核糖核酸酶I溶液已被用于消化各种样品中的DNA。
General description
脱氧核糖核酸酶Ⅰ将单链和双链 DNA 消化为带 5′磷酸盐和3′羟基末端的单核苷酸和寡核苷酸混合物。这种催化活性依赖于二价离子。在存在Mg2+的情况下，脱氧核糖核酸酶Ⅰ随机而独立地水解双链DNA的每条链。在存在Mn2+的情况下，两条链都可以被切开。
Other Notes
DNase I 溶于 50% (v/v) 甘油、20 mM 醋酸钠 (pH 6.5)、5 mM CaCl2 和 0.1 mM PMSF 溶液中。
使用小牛胸腺DNA作为底物时，一单位在每毫升反应混合物中每分钟可引起0.001的ΔA260。
用这种DNase I处理过的RNA不应该用于生成cDNA文库或用于RT-PCR。对于这些敏感的应用，建议使用扩展级DNase I，目录号AMP-D1。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Umesh Kumar Dhawan et al.
Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)
Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
商品
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
实验方案
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
相关内容
Instructions
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