一般描述
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
应用
- 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
- 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
- 从牛血样中提取的总 RNA 中去除污染 DNA
特点和优势
- 适用于去除 RNA 中的 DNA
- 超低RNase活性
- 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活
适用性
单位定义
法律信息
抑制剂
相关产品
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the concentration of the DNAse in Product AMPD1, DNase I?
The DNAse is provided at 1 unit/uL in 1 mL total volume.
What do I do if my DNA is not completely digested after 15 minute digestion at room temperature when using Product AMPD1, DNase I?
If further digestion is required, incubation can be performed for 30 minutes at 37°C.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
相关内容
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
PCR是分子生物学的核心技术，功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门