KlenTaq^® LA DNA Polymerase Mix

This polymerase mix contains Taq polymerase containing an N-terminal deletion combined with a small amount of a proofreading polymerase to improve sequence fidelity and length of amplified DNA.

KlenTaq^® LA DNA Polymerase Mix has been used to perform polymerase chain reaction (PCR). It has also been used to amplify FRD3 alleles from genomic DNA.

KlenTaq^® LA DNA Polymerase provides an excellent alternative to Taq DNA Polymerase for intermediate length products. It allows higher yields and greater fidelity (up to 4× that of Taq). It can amplify genomic DNA up to 5 kb or less complex DNA targets such as bacterial, viral targets or cDNA up to 20 kb. With its increased thermostability, it is the ideal choice for amplifying GC-rich regions or templates with difficult secondary structure.

KlenTaq LA DNA polymerase is provided with an optimized 10× reaction buffer.

KlenTaq LA DNA Polymerase Mix is an optimized mixture combining KlenTaq-1 with a proofreading enzyme. KlenTaq-1 is a Klenow-fragment analog of Taq DNA Polymerase. It has no endonuclease or exonuclease activity, but is more thermostable than Taq or other terminal deletions of Taq. Since a wide range of magnesium concentration is tolerated by this enzyme, generally no magnesium optimization is needed. The proofreading polymerase provides the 3′→5′ exonuclease activity that is necessary for longer and higher fidelity products.

One unit will incorporate 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

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KlenTaq is a registered trademark of Wayne Barnes