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安全信息

D0564

Sigma-Aldrich

Diphtheria Toxin

from Corynebacterium diphtheriae, ≥98% (SDS-PAGE), lyophilized powder, protein synthesis inhibitor

别名:

DTx

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About This Item

MDL编号:
MFCD00163490
UNSPSC代码:
12352200
NACRES:
NA.77

product name

白喉毒素 来源于白喉杆菌, lyophilized powder, Product is in unnicked form

质量水平

200

形式

lyophilized powder

储存温度

2-8°C

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一般描述

白喉毒素可用作细胞特异性细胞毒性免疫毒素中的毒素成分。具有天然内体逃逸机制的细菌毒素易位结构域已被用于开发有效的非病毒载体用于基因治疗。

应用

白喉棒状杆菌的白喉毒素已用于：


  • 小胶质细胞耗竭，研究小鼠创伤后应激障碍
  • 腹腔注射转基因小鼠，以在消融过程中融合移植的造血干细胞和祖细胞（HSPCs）后选择杂交种
  • 研究其对嗜酸性粒细胞缺陷小鼠株（iPHIL）中嗜酸性粒细胞系祖细胞的影响

生化/生理作用

白喉毒素是一种由白喉棒状杆菌产生的细菌毒素。它包含三个具有细胞内作用的结构域。这些结构域参与细胞中毒、细胞表面结合和内化为内体、穿过内体膜转运到胞质溶胶并抑制细胞蛋白质合成。白喉毒素可用于构建生物技术工具和治疗方法。它通过催化真核生物中靶蛋白的二磷酸腺苷（ADP）核糖基化来抑制蛋白质合成。

外形

含Tris和EDTA的冻干粉。

重悬

当用无菌蒸馏水将每管重悬至0.5 mL时，0.01 M Tris和0.001 M Na2EDTA，pH 7.5中含有~1 mg的白喉毒素。

象形图

Skull and crossbones
GHS06

警示用语：

Danger

危险声明

H300 + H330

危险分类

Acute Tox. 1 Inhalation - Acute Tox. 1 Oral

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--毒素类产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the solubility of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    This product is soluble in water.  Reconstitute with the volume of water described under Description on our website page for D0564.  

  4. What is the solution stability of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    Once the product is reconstituted in water, the solution may be stored in aliquots at -20°C. Avoid freeze-thawing. Long-term storage in the refrigerator at 2-8°C is not recommended.

  5. How stable is Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae, at low concentration?

    For assays employing low concentration of the toxin, the use of a carrier protein like serum albumin is recommended.   

  6. What is the molecular weight of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?

    In the presence of a reducing agent, on SDS gels, this preparation migrates as a major band of approx. mw 63,000 Daltons (corresponding to thte intact toxin) and two faster more lightly stained bands of approx. MW 39,000 and 24,000 Daltons (corresponding to the B and A fragments, respectively). Based on this method, the extent of the degree of nicking in this preparation is estimated to be less than 5%.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. How is Product D0564-1MG, Diphtheria Toxin, packaged?

    Product D0564-1MG, Diphtheria Toxin, is packaged in a small serum vial with a rubber or silicone septum and aluminum crimp top. The product can be reconstituted in the vial by piercing the septum with a syringe fitted with a stainless steel needle.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The Journal of Infectious Diseases, 181 (2000)
Diphtheria toxin
The Comprehensive Sourcebook of Bacterial Protein Toxins (2015)
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