product name
破伤风毒素 来源于破伤风梭菌,
分子量
150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
质量水平
溶解性
H2O: soluble
储存温度
2-8°C
基因信息
Clostridium tetani E88 ... tetX(1061100)
一般描述
Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.
应用
Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.
生化/生理作用
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.
制备说明
Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.
重悬
Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.
分析说明
Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).
警示用语：
Danger
危险声明
危险分类
Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
靶器官
Central nervous system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
法规信息
新产品
Tetanus
Hunter's Tropical Medicine and Emerging Infectious Disease, 508-510 (2013)
Felix Horns et al.
Cell reports, 30(3), 905-913 (2020-01-23)
Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and
MODELS vert The Tetanus Toxin Model of Temporal Lobe Epilepsy
Endocrine regulations, 804-807 (2009)
Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of alpha-synuclein via exosome
Yang Y, et al.
PLoS ONE, 12(7), e0180892-e0180892 (2017)
Rodolfo M Iglesias et al.
Neurochemical research, 37(6), 1355-1363 (2012-02-24)
Pannexin1 (Panx1), a protein related to the gap junction proteins of invertebrates, forms nonjunctional channels that open upon depolarization and in response to mechanical stretch and purinergic receptor stimulation. Importantly, ATP can be released through Panx1 channels, providing a possible
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