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Merck
CN
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安全信息

C4963

Sigma-Aldrich

过氧化氢酶-聚乙二醇

lyophilized powder, ~40,000 units/mg protein

别名:

PEG-过氧化氢酶

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About This Item

MDL编号:
MFCD00676653
UNSPSC代码:
12352204
NACRES:
NA.54

形式

lyophilized powder

质量水平

200

比活

~40,000 units/mg protein

分子量

PEG 5,000

组成

Protein, ~50% E405

标记范围

~40 mol PEG per mol protein

基质附着

secondary amine linkage.

储存温度

−20°C

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应用

经 PEG-过氧化氢酶处理的肺动脉内皮细胞可有效抑制 20-HETE（羟基二十碳四烯酸）诱导的荧光增强。但实验排除了 DCF（二氯荧光素）潜在的非特异性荧光。本实验研究了 20-HETE 对超氧化物产生和 NADPH 氧化酶活化的影响。

生化/生理作用

来自牛肝的过氧化氢酶催化 H 2 O 2 分解为水和氧。是一种四聚体，由分子量为 60 kDa 的四个相等的亚单位组成。每个亚基均含有与血红素 IX 基团结合的铁。该酶还能与靠近血红素基团的 NADP 强烈结合。过氧化氢酶活性在 pH 4.0-8.5 范围内恒定。pI 为 5.4。3-氨基-1-H-1,2,4 三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、联茴香胺和硝酸盐可抑制酶活性。用抗坏血酸或抗坏血酸/Cu 2 + 孵育过氧化氢酶会导致过氧化氢酶分子的降解。它不需要任何激活剂。

包装

基于蛋白质含量的包装规格

其他说明

来自牛肝脏的过氧化氢酶偶联甲氧基-聚乙二醇。

外形

含 PEG 加 5% 柠檬酸盐缓冲盐

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What can I use to solubilize Catalase-polyethylene glycol, Product C4963?

    It is soluble in water at a concentration of 1 mg/mL.

  4. What is the specific activity of Catalase-polyethylene glycol, Product C4963?

    The certificate of analysis on our website will have lot-specific activity information.

  5. What is the solution stability of Catalase-polyethylene glycol, Product C4963?

    We generally recommend preparing fresh solutions of Catalase-PEG. If stock solutions must be stored, we recommend first dissolving in water, then diluteing with an equal volume of glycerol. This 50% glycerol solution may be kept in the freezer at -20°C. Aqueous solutions held at 37°C were found to lose approximately half of the catalase activity after about 10 days.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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