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Merck
CN
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文件

安全信息

C9322

Sigma-Aldrich

过氧化氢酶 来源于牛肝脏

lyophilized powder, 2,000-5,000 units/mg protein

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别名:
H2O2:H2O2 氧化还原酶
CAS号:
9001-05-2
EC 号:
1.11.1.6 (BRENDA, IUBMB)
EC 号:
232-577-1
MDL编号:
MFCD00081483
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物来源

bovine liver

质量水平

400

形式

lyophilized powder

比活

2,000-5,000 units/mg protein

分子量

tetramer ~250 kDa

等电点

5.4

UniProt登记号

P00432

运输

wet ice

储存温度

−20°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

InChI key

NUVBSKCKDOMJSU-UHFFFAOYSA-N

基因信息

cow ... CAT(280743)

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应用

过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。

生化/生理作用

本品不需要任何活化剂，但其活性可被3-氨基-1-H-1,2,4三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、邻联茴香胺和硝酸盐抑制。
过氧化氢酶催化过氧化氢分解成水和氧气。 它还能与烷基过氧化氢反应，如甲基过氧化物和乙基过氧化物，可以用甲醇、乙醇、丙醇、甲酸盐和硝酸盐作为氢供体取代第二 H2O2 分子。

组分

牛肝脏过氧化氢酶是由4个相同亚基组成的四聚体，每个亚基的分子量为60kDa。 每个亚基都含有一个正铁血红素IX基团以及与之结合的铁。 过氧化氢酶也能紧密结合NADP，其中NADP和血红素基团的距离在13.7埃之内。

注意

过氧化氢酶溶液不可冷冻。 冷冻溶液将导致酶活性损失50-70％。

单位定义

一单位酶可在pH7.0、25℃下每分钟分解1.0 μM的H2O2，通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。

制备说明

该产品是一种冻干粉，活性是2,000-5,000单位/mg。
该酶溶于50 mM磷酸钾缓冲液中，浓度为1 mg/mL，pH 7.0。

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can Catalase from bovine liver, Product C9322, be reconstituted?

    This product is soluble in 50 mM potassium phosphate, pH 7.0 at a concentration of 10 mg/mL.

  4. How can a stock solution of Catalase from bovine liver, Product C9322, be stored?

    A 10 mg/mL stock solution prepared in 50 mM potassium phosphate, pH 7.0 and 50% glycerol was stable for at least 1 week in the freezer at -20°C.

  5. What salts are present in Catalase from bovine liver, Product C9322?

    Catalase (C9322) contains potassium phosphate buffered salts (and 20% trehalose as a stabilizer).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Ahmet H Kaya et al.
Brain research, 1221, 93-97 (2008-06-21)
Serotonin (5-HT) containing neurons in the dorsal raphe nucleus (DRN) may play important roles in Parkinson's disease (PD). This study investigated neural and metabolic activity of the DRN in animal PD models based on dopamine depletion. The data show both
Julie Elizabeth Keeble et al.
Pain, 141(1-2), 135-142 (2008-12-09)
Inflammatory diseases associated with pain are often difficult to treat in the clinic due to insufficient understanding of the nociceptive pathways involved. Recently, there has been considerable interest in the role of reactive oxygen species (ROS) in inflammatory disease, but
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商品

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

线粒体应激和ROS

氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。

实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

过氧化氢酶的酶学测定（EC 1.11.1.6）

过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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