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Merck
CN
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文件

安全信息

A2942

Sigma-Aldrich

Amphotericin B

solution, suitable for cell culture, BioReagent

别名:

Amphotericin, Fungizone

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About This Item

经验公式（希尔记法）:
C47H73NO17
CAS号:
1397-89-3
分子量:
924.08
MDL编号:
MFCD00877763
UNSPSC代码:
12352207
PubChem化学物质编号:
24890711
NACRES:
NA.76

product name

两性霉素 B 溶液, 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture

质量水平

200

无菌性

0.1 μm filtered

产品线

BioReagent

形式

liquid

浓度

250 μg/mL in deionized water

技术

cell culture | mammalian: suitable

颜色

yellow

抗生素抗菌谱

fungi
yeast

作用机制

cell membrane | interferes

运输

dry ice

储存温度

−20°C

SMILES字符串

[H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(=O)O[Na])O2

InChI

1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1

InChI key

APKFDSVGJQXUKY-INPOYWNPSA-N

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相关类别

一般描述

两性霉素B是一种大环内酯，具有亲水性多羟基链和亲脂性多烯烃链。

应用

两性霉素B溶液已被用于：
  • 作为腹腔注射剂用于治疗烟曲霉感染小鼠
  • 作为中国美利奴绵羊卵巢培养的理想培养基的组成部分
  • 在收集马黄体时用作运输培养基中的组成成分
  • 作为RPMI培养基中的补充剂，用于培养小鼠-小鼠B细胞杂交瘤55-6细胞系

生化/生理作用

两性霉素B对真菌和酵母最有效。它通过结合与膜相关的固醇而形成跨膜通道，从而导致小分子的泄漏。尽管两性霉素B是治疗真菌病的有效抗生素，它会引起毒性。
两性霉素B是一种来自链霉菌的多烯抗真菌抗生素，临床上用于治疗系统性真菌感染。它对真菌和细菌细胞膜的固醇，尤其是麦角固醇具有很高的亲和力。 维持抗真菌细胞培养的正常用量为2.5 mg/L，同时在培养基中使用青霉素和链霉素。

包装

20、50、100 ml

组分

该产品以250 μg/mL溶于去离子水中的两性霉素B提供。它是一种含有两性霉素B和以下提高其溶解度的组分组成的溶液：脱氧胆酸钠、氯化钠和磷酸钠。

注意

两性霉素B在37°C的培养条件下可保持活性3天。

制备说明

其在水溶液中形成沉淀是正常现象。 对溶液进行混合可重新溶解大部分沉淀物，但是，如果无法完全溶解，两性霉素B仍然适合使用。

其他说明

保存于密闭容器内，置于干燥通风处。光敏感。

通常也和此产品一起购买

产品编号
说明
价格

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product A2942, Amphotericin B solution, be stored?

    This product should be stored at -20 °C.  It is stable in culture at 37 °C up to 3 days.

  4. At what concentration should Product A2942, Amphotericin B solution, be used?

    We suggest a working concentration of 10 ml/L (2.5 mg/L).

  5. What is the shelf life of Product A2942, Amphotericin B solution?

    It has an expiration date of 18 months. This can be found on the lot-specific certificate of analysis.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

María Lourdes Acosta et al.
Cytotechnology, 54(3), 189-200 (2008-11-13)
Batch cultures were carried out to study the kinetic, stoichiometry, and regulation of glucose and glutamine metabolism of a murine hybridoma line. Asymmetric logistic equations (ALEs) were used to fit total and viable cell density, and nutrient and metabolite/product concentrations.
Yong-Hong Wang et al.
The Journal of antibiotics, 63(9), 553-557 (2010-07-08)
A new derivative of amphotericin B named amphotericin B(5) has been obtained under the following condition: amphotericin B powder was first dissolved in N,N-dimethylformamide at room temperature to obtain a solution of concentration of 2.0 mg ml(-1). Then, the solution
J Bolard et al.
Biochemistry, 30(23), 5707-5715 (1991-06-11)
The inducement of K+ permeability through membranes by the polyene antibiotic amphotericin B (AmB) has been analyzed as a measure of the antibiotic activity. Dose-response curves have been obtained with cholesterol- and ergosterol-containing egg yolk phosphatidylcholine large unilamellar vesicles (LUVs)
Antonio M Galvao et al.
Biology of reproduction, 83(6), 901-908 (2010-08-20)
Proapoptotic factor Fas ligand (FASL) and its cell surface receptor FAS are tumor necrosis factor superfamily members that trigger apoptosis in different cell types. However, their influence on luteal steroidogenesis is not clearly understood. The aim of the present work
Luigina Romani et al.
Blood, 103(11), 4232-4239 (2004-02-26)
Dendritic cells (DCs) show a remarkable functional plasticity in the recognition of Aspergillus fumigatus and orchestrate the antifungal immune resistance in the lungs. Here, we show that thymosin alpha 1, a naturally occurring thymic peptide, induces functional maturation and interleukin-12
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