Mpox Assay Development
In August of 2024, the World Health Organization (WHO) declared a global health emergency in response to the spread of mpox. The WHO has issued a call-to-action to develop a deeper understanding of viral transmission and molecular epidemiology, to address knowledge gaps in study design of vaccine efficacy, and to control outbreaks through the development of novel therapeutic treatments and vaccines. As a life science supplier, we are committed to providing reagents and technologies in support of scientists working to address this international health crisis. Explore our offering of products below.
Expediting Your Mpox Response, Vaccine Research, and Therapeutic Development
As of now, mpox continues to be monitored globally, with cases reported in various regions. Public health organizations are actively engaged in surveillance and response efforts to manage the spread of the virus. The focus remains on understanding transmission dynamics and implementing effective prevention strategies.
Innovative diagnostic solutions, such as lateral flow assay tests, are being developed to enhance the detection of mpox. These tests offer a rapid and efficient method for identifying the virus, facilitating timely responses in clinical and field settings. Researchers are working diligently to ensure these assays meet the necessary performance standards for accuracy and reliability.
To support the development of lateral flow assay tests for mpox detection, we emphasize the importance of high-quality raw materials and research products. Our offerings include essential components that are critical for the successful creation of these assays. By providing the necessary resources, we aim to empower researchers and developers in their efforts to combat mpox effectively.
For inquiries about our mpox-related products or to learn more about our offerings, our Technical Experts are ready to assist you.
Lateral Flow Membrane
Hi-Flow™ Plus membranes are the membrane of choice for diagnostic tests and kits, offering superior consistency and performance.
Standard (20 mm & 25 mm) and customized slit widths of Hi-Flow™ Plus membranes are also available to order offline as master rolls. Each master roll is 30.5 cm wide and 100 m in length. For ease of use, master rolls are cut into multiple ready-to-use slit rolls at the desired width prior to delivery. The number of slit rolls received per master roll order is tied to the required width of the slit roll. As an example, an order for 1 master roll with a required slit width of 18 mm will yield 16 slit rolls.
Please contact your local account manager or our customer service team to obtain a catalog number for your membrane and slit width of choice.
Antibodies for Serology Assay Development
Antibody product range useful for subclassification of human immunoglobulins (IgM, IgG, IgA) in whole blood for serological assay development.
For Research Use Only.
Microspheres & Nanoparticles
Estapor® Microspheres are a leading brand of polymeric supports for in vitro diagnostics, life sciences, biotechnology and environmental applications. We manufacture, develop, produce, and provide more than 100 different types of microspheres worldwide.
For Research Use Only. Not for Use in Diagnostic Procedures.
Gold Nanoparticles
Gold nanoparticles (20-50 nm) and our gold nanoparticle kits provide an easy way to customize your colorimetric lateral flow test to your antibody of choice. Gold nanoparticles are chemically inert, have a long shelf life, and exhibit exceptional optical properties, making them one of the most widely used choices for Lateral Flow Assays. Download our Bioconjugation Handbook.
For Research Use Only. Not for Use in Diagnostic Procedures.
Probumin
Probumin Bovine Serum Albumin is for protease sensitive immunoassays and as a stabilizing agent for protein reagents requiring low IgG levels; may be used for molecular biology applications. Purity is ≥ 98%; typical purity is 100%.
ProClin™ 300
Biocide designed specifically for use within Diagnostics manufacturing applications only. An effective, environmentally-friendly alternative to existing diagnostic reagent preservatives. At recommended usage levels, ProClin™ presents no health hazards, toxicological problems, or disposal issues.
For Research Use Only.
Ultrapure Water
With rapidly evolving and diverse research projects, changing priorities, multiple users, and limited space, it’s critical for laboratories to select equipment that is versatile, innovative and flexible to their space requirements. Our Milli-Q® IQ 7000 ultrapure water system and its application filters tick all these boxes.
Key Components for Viral Transport Media
The CDC recently published method for labs to create their own viral transport media. Refer to SOP#: DSR-052-01: Preparation of Viral Transport Media. Specimens can be stored for up to 72 hours at 4°C.
For Research Use Only.
Accelerating Your Research with Tools for Viral DNA Extraction, Purification, and qPCR
To support the development of molecular assays for mpox detection, our offerings include reagents and kits for viral DNA extraction and purification, enzymes for qPCR amplification, and custom primers and probes. Explore our tools and configurators for enzyme selection and probe design for optimal results.
Viral DNA Extraction and Purification Kits
Extraction of nucleic acids from samples is critical for viral detection assays. GenElute™-E Viral RNA/DNA kits provide quality nucleic acids with an efficient protocol, saving time and reducing plastic use for a more sustainable system. Obtain purified viral nucleic acids in less than 5 minutes. For assays requiring silica-based purification, GenElute™ Viral RNA miniprep kit is designed for isolation of viral RNA in a column-based format.
For Research Use Only. Not for Use in Diagnostic Procedures.
qPCR Amplification
qPCR DNA Amplification Enzymes
Explore the extensive range of qPCR amplification enzymes for assay development.
For Probe-Based Assays*
LuminoCt® qPCR ReadyMix™
LuminoCt® qPCR ReadyMix™ is designed specifically for rapid, two-step qPCR protocols that deliver results in as little as 25 minutes. This is the ideal solution for performing high-throughput, quantitative PCR methods that rely on a fluorescent probe. With a separate ROX internal reference dye, the master mix is compatible with all commonly available real-time instrument platforms.
KiCqStart® Probe qPCR ReadyMix™
KiCqStart® qPCR Master mix features a stringent hot-start mechanism for greater specificity. The master mix contains all required basic components for qPCR; simply add primers, probe, and water to finish preparing your assay. KiCqStart® is optimized with ROX based on instrument requirement. Suitable for probe-based detection methods, the product line features options for optimization and offers compatibility with tube-, plate-, and capillary-based instruments.
FastStart™ Universal Probe Master
FastStart™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format.
EagleTaq Universal Master Mix (ROX)
EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) hot start master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) hydrolysis probe reactions. It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
KAPA PROBE FORCE
KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.
KAPA PROBE FAST
KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).
For SYBR® Green-Based Assays*
KiCqStart® SYBR® Green qPCR ReadyMix™
KiCqStart® SYBR® Green qPCR ReadyMix™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR). This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR sensitivity, specificity, and efficiency with assay results in as little as 33 minutes, yielding a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.
LuminoCt® SYBR® Green qPCR ReadyMix™
LuminoCt® SYBR® Green qPCR ReadyMix™ is a ready-to-use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. It is ideal for performing fast, high-throughput, SYBR Green-based quantitative PCR applications on commonly available real-time instrument platforms.
FastStart™ SYBR® Green Master
FastStart™ SYBR® Green Master is a ready-to-use hot start reaction mix for quantitative polymerase chain reaction (qPCR). This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart™ TaqMan® Probe Master allows very sensitive detection and quantification of defined DNA sequences.
KAPA SYBR® FAST
KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR® FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.
KOD One™ PCR Master Mix
KOD One™ PCR Master Mix is the right choice for ultra-fast PCR and qPCR. It offers the fastest elongation rates available for high fidelity polymerases without compromising on the yield and specificity. This convenient, premixed, ready-to-use solution offers an elongation rate of up to 5 sec/kb and exhibits approximately 80-fold higher fidelity than Taq DNA polymerase.
*KAPA products are not available in the United States and Japan. Please go to sequencing.roche.com to purchase directly.
Product Selection Guide | |||||
|---|---|---|---|---|---|
Product Name | Prod. No. | SYBR® Green Reagent | Probe-Based | ROX™ Reagent | Instrument Compatibility |
KiCqStart® SYBR® Green qPCR ReadyMix™ Reagent | ✔ | Bio-Rad, Cepheid®, Eppendorf, Illumina, Corbett, and Roche systems | |||
Low ROX™ Reagent | ABI and StrataGene® instruments | ||||
With ROX™ Reagent | ABI instruments | ||||
iQ™, with fluorescein for Bio-Rad systems | |||||
KiCqStart® Probe qPCR ReadyMix™ Reagent | ✔ | For Bio-Rad, Cepheid®, Eppendorf, Illumina, Corbett, and Roche systems | |||
Low ROX™ Reagent | with Low ROX for ABI and StrataGene® instruments | ||||
With ROX™ Reagent | with ROX for ABI instruments | ||||
LuminoCt® qPCR ReadyMix™ Reagent | ✔ | Separate reference dye | Compatible with any qPCR instrument. Select the proper final ROX concentration based on your instrument. | ||
✔ | |||||
SYBR® Green JumpStart™ Taq ReadyMix™ Reagent | ✔ | Separate reference dye | Compatible with any qPCR instrument. Select the proper final ROX concentration based on your instrument. | ||
JumpStart™ Taq ReadyMix™ for Quantitative PCR |
| ✔ | Separate reference dye | Compatible with any qPCR instrument. Select the proper final ROX concentration based on your instrument. | |
JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR |
| ✔ | Premixed reference dye | ABI instruments and StepOne™ | |
Custom Primers and Probes
The outbreak of mpox has increased the demand for primers and probes for qPCR-based detection. Leverage our 35+ years of experience and global supply chain for expertly manufactured and reliably delivered oligos.
Useful mpox oligonucleotide resources can be found on this web page: NIST Mpox (MPXV) Synthetic DNA PCR Standards
Copy and paste the desired sequences from the above webpage into the configurators below to order in real time.
If you have needs that are different from the other general specifications presented, please send a request to dnaoligos@milliporesigma.com.
Products & Specifications | |
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| Order Dual-Labeled Probes*
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*The probes from the U.S. CDC assays are labeled with internal BHQ-1-dT and have a terminal phosphate or spacer to prevent extension of the probe. Customers routinely convert the internal BHQ to a 3’ BHQ for simplicity and find performance to be suitable. In this case, the above Custom Dual-Labeled Probes ordering configurator can be used to order in real time. If you require the internal BHQ, please send an email to the address above to receive a quotation.
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