生物来源
goat
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
形式
buffered aqueous solution
种属反应性
rabbit
浓度
~1 mg/mL
荧光
λex 638 nm; λem 651 nm in PBS, pH 7.4
储存温度
−20°C
一般描述
Abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by Abberior: the class of phosphorylated fluorescent dye. The dye excels in its photophysical parameters: it is very bright, very stable and it enables basically background free imaging. The dye works exceptionally well with Abberior Instruments STEDYCON und Expert line microscopes and Leica STED microscopes. Abberior STAR 635P can substitute Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR 635P is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
适用性
Designed and tested for fluorescent super-resolution microscopy
分析说明
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
法律信息
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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