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表单
solid
溶解性
DMF: 0.25 mg/mL, clear
荧光
λex 634 nm; λem 654.0 nm±15 nm in PBS (pH 7.1)
适用性
suitable for fluorescence
储存温度
−20°C
一般描述
Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED packages.
Key Features
Absorption Maximum, λmax: 633 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75′000 M-1cm-1 (water)
Correction Factor, CF260 = ε260/εmax: 0.42
Correction Factor, CF280 = ε280/εmax: 0.47
Fluorescence Maximum, λfl: 653 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.92 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 (PBS, pH 7.4)
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED packages.
Key Features
- First phosphorylated dye
- Unmatched, background free STED imaging contrast
- Verified in the Leica STED system
Absorption Maximum, λmax: 633 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75′000 M-1cm-1 (water)
Correction Factor, CF260 = ε260/εmax: 0.42
Correction Factor, CF280 = ε280/εmax: 0.47
Fluorescence Maximum, λfl: 653 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.92 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 (PBS, pH 7.4)
适用性
Designed and tested for fluorescent super-resolution microscopy
法律信息
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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