推荐产品
生物来源
goat
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
种属反应性
rabbit
存货情况
available only in USA and Canada
浓度
~1 mg/mL
荧光
λex 604 nm; λem 623 nm in PBS, pH 7.4
储存温度
−20°C
一般描述
Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto™ 590, Atto™ 594 or Alexa Fluor™ 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 604 (PBS pH 7.4), 604 (H2O), 601 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: : 95 000 (PBS pH 7.4), 95 000 (H2O), 100 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,25 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 623 (PBS pH 7.4), 623 (H2O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 604 (PBS pH 7.4), 604 (H2O), 601 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: : 95 000 (PBS pH 7.4), 95 000 (H2O), 100 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,25 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 623 (PBS pH 7.4), 623 (H2O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)
适用性
Designed and tested for fluorescent super-resolution microscopy
分析说明
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
法律信息
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Tobias Müller et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(8), 1986-2000 (2012-03-01)
For about a decade, superresolution fluorescence microscopy has been advancing steadily, maturing from the proof-of-principle stage to routine application. Of the various techniques, STED (stimulated emission depletion) microscopy was the first to break the diffraction barrier. Today, it is a
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