产品名称
抗-小鼠-IgG - Atto 647N 山羊抗, contains 50% glycerol as stabilizer
conjugate
Atto 647N conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol as stabilizer
species reactivity
mouse
concentration
≥0.8 mg/mL IgG
technique(s)
immunofluorescence: 5 μg/mL
fluorescence
λex 647 nm; λem 665 nm in PBS
suitability
in accordance for fluorescence
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
未缀合染料的总荧光 ≤5%
Application
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性，构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越，光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗（浓度为 5μg/ml），用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析（1 篇论文）
蛋白质免疫印迹分析（1 篇论文）
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
免疫球蛋白G（IgG）是一种糖蛋白抗体，其可调节免疫反应，如吞噬作用，也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型，即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。Atto 647N山羊抗小鼠IgG与小鼠IgG结合。
Immunogen
小鼠 IgG
Legal Information
本产品仅供研究使用。如果要商业化，请联系知识产权持有者（ATTO-TEC GmbH，德国）获取许可。
Physical form
Atto 647 山羊抗小鼠 IgG（全分子）以 1 mg/ml 溶液的形式提供，溶剂为 0.1M 磷酸钠，0.1M NaCl，pH 7.5，含有 5 mM 叠氮化钠作为防腐剂。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
Zoran V Popovic et al.
Scientific reports, 7(1), 311-311 (2017-03-24)
Tissue osmolarity varies among different organs and can be considerably increased under pathologic conditions. Hyperosmolarity has been associated with altered stimulatory properties of immune cells, especially macrophages and dendritic cells. We have recently reported that dendritic cells upon exposure to
Beatriz Marcos-Ramiro et al.
The Journal of cell biology, 213(3), 385-402 (2016-05-04)
Endothelial barrier dysfunction underlies chronic inflammatory diseases. In searching for new proteins essential to the human endothelial inflammatory response, we have found that the endosomal GTPase RhoB is up-regulated in response to inflammatory cytokines and expressed in the endothelium of
Audrey Durand et al.
Nature communications, 9(1), 5247-5247 (2018-12-12)
Traditional approaches for finding well-performing parameterizations of complex imaging systems, such as super-resolution microscopes rely on an extensive exploration phase over the illumination and acquisition settings, prior to the imaging task. This strategy suffers from several issues: it requires a
H Singh et al.
Neuroscience, 317, 76-107 (2016-01-17)
Large conductance voltage and calcium-activated potassium (MaxiK) channels are activated by membrane depolarization and elevated cytosolic Ca(2+). In the brain, they localize to neurons and astrocytes, where they play roles such as resetting the membrane potential during an action potential
Jeffrey M Schaub et al.
Langmuir : the ACS journal of surfaces and colloids, 34(49), 14882-14890 (2018-07-26)
Single-stranded DNA (ssDNA) is a critical intermediate in all DNA transactions. Because ssDNA is more flexible than double-stranded (ds) DNA, interactions with ssDNA-binding proteins (SSBs) may significantly compact or elongate the ssDNA molecule. Here, we develop and characterize low-complexity ssDNA
商品
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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