LX-2 Cas9 Human Hepatic Stellate Cell Line

Hepatic stellate cells are a cell type responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. Given the importance of stellate cells in disease etiology, genetically malleable cellular models are critical for understanding the contribution of these cells to liver pathologies and necessary for development of new therapeutics.

The immortalized LX-2 line was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

Source
LX-2 Cas9 human hepatic stellate cells were derived from LX-2 cells transformed with a Cas9 cassette.
LX-2 was generated by immortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passage cells in low serum media conditions.

References
1.Gut 2005, 54(1): 142-151.
2.PloS One 2013, 8(10): e75692.

The LX-2 Cas9 human hepatic stellate cell line has been extensively characterized and retains key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making it a highly suitable model of human hepatic fibrosis.

LX-2 Cas9 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and functionality. LX-2 cells have been successfully expanded beyond passage 50 in culture.

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