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SCC300

3dGRO^® Human iPSC Derived Colon Organoids

Name: 3dGRO<SUP>®</SUP> Human iPSC Derived Colon Organoids
Brand: MM

别名:

Human Colon Organoids, Human Colonoids, Human Gut Organoids, Human Intestinal Organoids

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About This Item

UNSPSC代码:

41106514

NACRES:

NA.81

产品SCC300监管中，请联系销售询价或购买联系客户支持

一般描述

Human intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).

3dGRO^® Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO^® human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO^® Human Colon Organoid Expansion Medium (Cat. No. SCM304).

应用

Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).

Research Category:

Cell Culture
Stem Cell Research

质量

Each vial contains ≥1500 organoids.
Mycoplasma: Negative
Genotyped by STR analysis: Pass
Human species verified
Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
Cell viability test: Pass

储存及稳定性

3dGRO^® Human iPSC Derived Colon Organoids should be stored in liquid nitrogen.

法律信息

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

高风险级别生物产品-Merck

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商品

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

实验方案

通过无血清细胞培养条件将人多能诱导干细胞(iPS)体外分化为结肠类器官

高度表征的冻存人结肠类器官，iPS细胞分化为肠道上皮类器官的分步方案。

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Forskolin-Induced Swelling Assays using Millicell^® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell^® Microwell plates and perform a forskolin-induced swelling assay.

此商品	SCC741	SCC742	SCC739
SCC300 3dGRO^® Human iPSC Derived Colon Organoids	SCC741 3dGRO^® Colorectal Organoids (CSC.413)	SCC742 3dGRO^® Colorectal Organoids (CSC.456)	SCC739 3dGRO^® Colorectal Organoids (CSC.171C.G1)
form liquid	form -	form -	form -
application(s) cell analysis	application(s) -	application(s) -	application(s) -

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