Anterior Foregut Endoderm Induction Medium II

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

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The Anterior Foregut Endoderm Induction Medium II is a chemically defined/serum-free ready-to-use media used to differentiate CXCR4⁺/c-kit⁺ definitive endoderm (DE) cells into Sox2⁺/Pax6⁺ anterior foregut endoderm (AFE) cells. The Anterior Foregut Endoderm Induction Medium II is a component of the 3dGRO^® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO^® Human Lung Organoid Culture System includes (all sold separately):

• Definitive Endoderm Induction Medium (SCM302)
• Anterior Foregut Endoderm Induction Medium I (SCM305)
• Anterior Foregut Endoderm Induction Medium II (SCM306)
• 3dGRO^® Lung Organoid Branching Medium (SCM307)
• 3dGRO^® Lung Organoid Maturation Medium (SCM308)

Human iPSC Derived AFE Progenitors (SCC301)