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GERPN3005

ECL Direct Nucleic Acid

Cytiva RPN3005, pack of 1 ea

Synonym(s):

DNA/RNA detection, HRP, chemiluminescent detection, luminol, module, reagent

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About This Item

NACRES:
NA.31
UNSPSC Code:
41105300
Storage temp.:
2-8°C
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packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3005

storage temp.

2-8°C

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General description

ECL Direct Labeling Module.

Application

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

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Storage Class

12 - Non Combustible Liquids

Regulatory Information

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Senia Rosales et al.
BMC microbiology, 10, 208-208 (2010-08-04)
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G Y Akita et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 5(2), 154-158 (1993-04-01)
A previously described bluetongue virus (BTV) serogroup polymerase chain reaction (PCR) assay was applied to clinical samples. The sensitivity of the BTV serogroup PCR was increased by the use of non-radioactive chemiluminescent hybridization. Unfractionated whole blood samples from rams experimentally
C Couturier et al.
Arteriosclerosis, thrombosis, and vascular biology, 20(12), 2559-2565 (2000-12-16)
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K Aogi et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(10), 2790-2797 (1999-10-28)
The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of
H M Peng et al.
Molecular pharmacology, 54(4), 740-747 (1998-10-10)
The regulation of cytochrome P450 (CYP) 2E1, the ethanol-inducible isoform, is particularly complex. The level is affected by a variety of other foreign compounds, by insulin (as studied in several laboratories), and by triiodothyronine (T3), which has not been previously

Articles

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。

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