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GERPN3001

ECL™ Direct Nucleic Acid

Cytiva RPN3001, pack of 1 ea

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Select a Size

10 G
¥1,590.46
100 G
¥9,752.37

¥1,590.46

Available to ship on2025年4月07日Details

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10 G
¥1,590.46
100 G
¥9,752.37

About This Item

UNSPSC Code:
41105300
NACRES:
NA.31

¥1,590.46

Available to ship on2025年4月07日Details

packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3001

storage temp.

2-8°C

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Show Differences

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This Item
G7021G5146G7528
D-(+)-Glucose suitable for mouse embryo cell culture, ≥99.5% (GC)

G6152

D-(+)-Glucose

D-(+)-Glucose powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

G7021

D-(+)-Glucose

D-(+)-Glucose Hybri-Max™, powder, BioReagent, suitable for hybridoma

G5146

D-(+)-Glucose

D-(+)-Glucose ≥99.5% (GC), BioXtra

G7528

D-(+)-Glucose

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

gas chromatography (GC): suitable

solubility

H2O: 2g + 15 mL, clear, colorless

solubility

H2O: 2g + 15 mL (Tested)

solubility

H2O: 2g + 15 mL (Tested)

solubility

H2O: 1 M

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

General description

ECL™ Direct Labeling and Detection System.

Application

ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.

Features and Benefits

  • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
  • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
  • No need to strip blots before reprobing.
  • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
  • Consistent results combining strong signals with very Low backgrounds.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

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Pictograms

Exclamation mark
GHS07

Signal Word

Danger

Storage Class Code

12 - Non Combustible Liquids

Regulatory Information

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    C Couturier et al.
    The Journal of biological chemistry, 274(33), 23085-23093 (1999-08-07)
    Type II-secreted phospholipase A(2) (type II-sPLA(2)) is expressed in smooth muscle cells during atherosclerosis or in response to interleukin-1beta. The present study shows that the induction of type II-sPLA(2) gene by interleukin-1beta requires activation of the NFkappaB pathway and cytosolic
    Detection of the antiseptic- and disinfectant-resistance genes qacA, qacB, and qacC in methicillin-resistant Staphylococcus aureus isolated in a Tokyo hospital.
    Jun-ichiro Sekiguchi et al.
    Japanese journal of infectious diseases, 57(6), 288-291 (2004-12-30)
    H M Peng et al.
    Molecular pharmacology, 54(4), 740-747 (1998-10-10)
    The regulation of cytochrome P450 (CYP) 2E1, the ethanol-inducible isoform, is particularly complex. The level is affected by a variety of other foreign compounds, by insulin (as studied in several laboratories), and by triiodothyronine (T3), which has not been previously
    K Aogi et al.
    Clinical cancer research : an official journal of the American Association for Cancer Research, 5(10), 2790-2797 (1999-10-28)
    The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of
    G Y Akita et al.
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 5(2), 154-158 (1993-04-01)
    A previously described bluetongue virus (BTV) serogroup polymerase chain reaction (PCR) assay was applied to clinical samples. The sensitivity of the BTV serogroup PCR was increased by the use of non-radioactive chemiluminescent hybridization. Unfractionated whole blood samples from rams experimentally
    View All Related Papers

    Articles

    Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

    Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service