biological source
chicken egg white
assay
≥95%
form
solid
specific activity
>23000 U/mg (Shugar units)
mol wt
14.4 kDa
packaging
pkg of 10 g
manufacturer/tradename
Roche
optimum pH
6.0-7.0
absorption
3.9 at 280 nm (10 mg enzyme/ml)
General description
溶菌酶，粘肽乙酰胞壁质聚糖水解酶
Application
溶菌酶已用于监测实时PCR的转录过程和样品制备。
溶菌酶已用于：
- 细胞壁降解
- 原生质体的制备
- 溶菌
- 药理学（对粘膜的抗炎作用；组织愈合；防止出血；粘液分泌正常化；热原溶解；增强抗生素作用）
- 食品和饮料（增味剂）
- 分离核酸前的样品制备
Biochem/physiol Actions
溶菌酶水解切割粘多糖和肽聚糖相应聚糖骨架（后者是与肽链连接或交联的糖胺聚糖）中N-乙酰-D-葡糖胺与N-乙酰胞壁酸残基（GlcNAc β1-4Mur）之间的键。溶菌酶能有效破坏细菌的细胞壁。
Preparation Note
2至8 °C储存。（干燥储存！）
工作溶液：盐酸溶菌酶易溶于水和缓冲溶液，但不溶于有机溶剂。
储存条件（工作溶液）：-15至-25°C
盐酸溶菌酶水溶液（2mg/ml，溶剂为蒸馏水）可在2至8℃下储存数天，或在-15至-25℃下储存数周。
储存条件（工作溶液）：-15至-25°C
盐酸溶菌酶水溶液（2mg/ml，溶剂为蒸馏水）可在2至8℃下储存数天，或在-15至-25℃下储存数周。
使用双蒸水制备浓度为50 mg/ml的储备溶液。将储备溶液分装并保存在-15至-25°C。分装品用后丢弃。
Other Notes
一个Shugar单位是指：在+25°C下，在1 ml pH7.0的溶壁微球菌( Micrococcus luteus )悬浮液中，在450nm波长处的初始吸光度为0.750，路径长度为10mm，引起吸光度以每分钟0.001的速率降低的酶量。
仅用于生命科学研究。不可用于诊断。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
动植物源性产品
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