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等级
for molecular biology
质量水平
形式
liquid
技术
DNA amplification: suitable
DNA extraction: suitable
储存温度
−20°C
一般描述
这种中和缓冲液B要与 Sigma′REDExtract-N-Amp™ 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液，并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B，每次提取至少可中和一次。 不过，如果需要更多的扩增反应，则可能需要补充额外的中和缓冲液。
应用
中和缓冲液B 已被用于改良的 Sigma Extract-N-Amp ™ 提取程序。它也用于小鼠的基因分型，用于聚合酶链反应 (PCR) 之前使用Sigma′的REDExtract-N-Amp™ PCR 反应混合物来中和DNA提取液。
法律信息
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Warning
法规信息
含少量动物源组分生物产品
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
Jun He et al.
Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
实验方案
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
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