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主要文件

安全信息

N3910

Sigma-Aldrich

中和液 B

别名:

DNA neutralization solution, neutralization buffer DNA extraction

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40 TESTS
CN¥2,985.95

CN¥2,985.95


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变更视图
40 TESTS
CN¥2,985.95

About This Item

UNSPSC代码:
12161703
NACRES:
NA.25

CN¥2,985.95


请联系客服了解存货情况

等级

Molecular Biology

质量水平

表单

liquid

技术

DNA amplification: suitable
DNA extraction: suitable

储存温度

−20°C

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此商品
T9281T22500V900853
assay

≥99% (GC)

assay

≥99.0%

assay

99%

assay

≥99%

grade

for molecular biology

grade

-

grade

-

grade

reagent grade

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

一般描述

这种中和缓冲液B要与 Sigma′REDExtract-N-Amp 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液,并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B,每次提取至少可中和一次。 不过,如果需要更多的扩增反应,则可能需要补充额外的中和缓冲液。

应用

中和缓冲液B 已用于改良 Sigma Extract-N-Amp提取程序。[1]它还用于小鼠的基因分型,在聚合酶链反应 (PCR) 之前可使用Sigma的REDExtract-N-Amp PCR 反应混合物来中和DNA提取液。[2]

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    Yan Zhang et al.
    American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
    Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
    Işın Çakır et al.
    Nature metabolism, 4(1), 44-59 (2022-01-19)
    The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
    A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
    Varshney GK, et al.
    Nature Protocols, 11(12), 2357-2357 (2016)
    Jun He et al.
    Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
    Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
    Yan Zhang et al.
    American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
    Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed

    实验方案

    SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

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