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11745832910

Roche

DIG-High Prime DNA标记及检测启动装I

greener alternative

sufficient for 12 labeling reactions, sufficient for 24 blots, suitable for hybridization, suitable for Northern blotting, suitable for Southern blotting

别名:

DIG系统, DNA标记

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5 G
¥880.73
25 G
¥4,173.76
100 G
¥8,121.25

¥880.73

国内现货，预计发货时间2025年4月27日详情

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5 G
¥880.73
25 G
¥4,173.76
100 G
¥8,121.25

About This Item

UNSPSC代码:
41105500

¥880.73

国内现货，预计发货时间2025年4月27日详情

用途

sufficient for 12 labeling reactions
 sufficient for 24 blots

质量水平

100

包装

kit of 1 (7 components)

制造商/商品名称

Roche

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技术

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

环保替代产品分类

, Aligned

储存温度

−20°C

相关类别

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form

powder

form

powder

form

powder

form

solid

λmax

534, 395, 312 nm (lit.)

λmax

531, 314, 295 nm (lit.)

λmax

534, 393, 313 nm (lit.)

λmax

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

assay

95% (NMR)

assay

95% (NMR)

assay

95% (NMR)

assay

96%

mp

>300 °C

mp

196 °C

mp

250-260 °C

mp

85-90 °C

一般描述

DIG High Prime DNA标记及检测起始试剂盒I使用类固醇半抗原地高辛(DIG)，通过酶促免疫检测标记DNA探针标记，以便进行杂交和后续显色检测。此试剂盒包含即用型封闭液——由硝基四氮唑蓝（NBT）/ 5-溴-4-氯-3-吲哚基磷酸酯(BCIP和DIG Easy Hyb颗粒组成的综合储备液。DIG-High Prime混合物包括稳定的Klenow酶、核苷酸、引物和反应缓冲液——所有成分均溶于一种方便的试剂中。样品材料可以是：至少100 bp的DNA片段，线性化质粒，cosmid或λ DNA，或超螺旋DNA“随机结合的”DNA标记方法首先由Feinberg和Vogelstein开发，基于将所有可能序列的寡核苷酸杂交至待标记的变性DNA。初始加入的DNA仅作为标记DNA的合成模板，在反应过程中不会降解，因而可标记极少量的DNA（10 ng）。该方法借助Klenow聚合酶，以随机寡核苷酸的3′-OH末端为引物，合成互补DNA链。反应物中存在洋地黄毒苷标记的修饰脱氧核糖核苷三磷酸掺入新合成的互补DNA链中。这种便捷的试剂盒可通过碱不稳定的DIG-11-dUTP随即引发标记DNA模板，显色检测DIG标记杂交产物。我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。  DIG系统是灵敏且具有成本效益的方案，可替代核酸放射性标记和检测。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。

应用

DIG-High Prime DNA标记和检测起始试剂盒I已用于多种杂交技术：
  • Southern印迹[1][2]
  • Nrthern印迹
  • 斑点印迹[3]
  • 菌落和噬菌斑杂交
  • 所有类型的过滤杂交
  • 总基因组DNA的单拷贝基因检测，甚至可检测高度复杂的生物体样品，如人类、大麦和小麦
一小时或过夜孵育后，DIG 标记的探针产量很高。

特点和优势

我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。 DIG系统是灵敏且具有成本效益的方案，可替代放射性标记和放射法检测核酸。现有的许多出版物证明了DIG系统的多功能性，因此使用放射性标记不再是杂交DNA标记的唯一选择。

包装

1个试剂盒包含7种组分。

质量

已通过斑点杂交试验测试功能。

其他说明

仅用于生命科学研究。不用于诊断操作。

仅试剂盒组分

产品编号
说明
  • DIG-High Prime 5x concentrated
  • DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml
  • DNA Dilution Buffer
  • Anti-Digoxigenin-AP Conjugate antibody
  • NBT/BCIP Stock Solution, concentrated
  • Blocking Solution 10x concentrated
  • DIG Easy Hyb Granules

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315,H319

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

含少量动物源组分生物产品
常规特殊物品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    67358120
    58598220
    56477320
    47754321
    35668922

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    商品

    Digoxigenin (DIG) Labeling Methods

    Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

    地高辛 (DIG) 标记方法

    地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。

    我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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