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D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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100 EA
CN¥6,144.32

CN¥6,144.32

Estimated to ship onApril 27, 2025Details

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100 EA
CN¥6,144.32

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

CN¥6,144.32

Estimated to ship onApril 27, 2025Details

Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

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This Item
C4767PHR1439C4930
β-Cyclodextrin powder, BioReagent, suitable for cell culture, ≥97%

C4805

β-Cyclodextrin

β-Cyclodextrin ≥97%

C4767

β-Cyclodextrin

Beta-Cyclodextrin Pharmaceutical Secondary Standard; Certified Reference Material

PHR1439

Beta-Cyclodextrin

γ-Cyclodextrin powder, BioReagent, suitable for cell culture, ≥98%

C4930

γ-Cyclodextrin

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

cell culture | mammalian: suitable

sterility

non-sterile

sterility

-

sterility

-

sterility

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

solubility

1 M NaOH: 50 mg/mL

solubility

1 M NH4OH: 50 mg/mL

solubility

-

solubility

1 M NaOH: 25 mg/mL, clear to slightly hazy

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000411631
0000411631
0000375543
SLCN0620
0000320236

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It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
M A Hull et al.
Gut, 43(4), 525-536 (1998-11-21)
Basic fibroblast growth factor (bFGF) promotes angiogenesis and healing of gastric ulcers in rats, and bFGF expression is up regulated in such ulcers. However, little is known about expression of bFGF in human gastric mucosa. To investigate bFGF expression in
J Stokes et al.
Investigative ophthalmology & visual science, 41(7), 1629-1638 (2000-06-14)
The administration of glucocorticoids as topical or systemic medications may lead to the development of ocular hypertension through the induction of morphologic and biochemical changes in the trabecular meshwork leading to a reduction in the facility of aqueous outflow. Glucocorticoids
Gregor Obernosterer et al.
Nature protocols, 2(6), 1508-1514 (2007-06-16)
Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in
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Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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