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GERPN3000

ECL™ Direct Nucleic Acid

Cytiva RPN3000, pack of 1 ea

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UNSPSC Code:
41105300
NACRES:
NA.31

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3000

storage temp.

2-8°C

Related Categories

General description

ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.

Application

ECL™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.

Features and Benefits

  • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
  • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
  • No need to strip blots before reprobing.
  • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
  • Consistent results combining strong signals with very Low backgrounds.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

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GERPN3001

ECL™ Direct Nucleic Acid, Cytiva RPN3001, pack of 1 ea

GERPN3004

ECL™ Detection Reagents, Cytiva RPN3004, pack of 1 ea

GERPN3005

ECL™ Direct Nucleic Acid, Cytiva RPN3005, pack of 1 ea

GERPN3006

ECL™ Gold Buffer, Cytiva RPN3006, pack of 1 ea

Pictograms

Exclamation mark
GHS07

Signal Word

Danger

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kostyantyn V Dmytruk et al.
BMC biotechnology, 11, 58-58 (2011-05-27)
The detection and quantification of uric acid in human physiological fluids is of great importance in the diagnosis and therapy of patients suffering from a range of disorders associated with altered purine metabolism, most notably gout and hyperuricaemia. The fabrication
The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
Leao SC, et al.
PLoS ONE, 8(4), e60746-e60746 (2013)
CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae.
Sasano Y and Harashima S
Bio-protocol, 7(10) (2017)
Elevated urinary level of vitamin D binding protein as a novel biomarker for diabetic nephropathy.
Tian XQ, et al.
Experimental and Therapeutic Medicine, 7(2), 411-416 (2014)
Transfer of antibiotic resistance marker genes between lactic acid bacteria in model rumen and plant environments.
Toomey N, et al.
Applied and Environmental Microbiology, 75(10), 3146-3152 (2009)
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Articles

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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