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Safety Information

N3910

Sigma-Aldrich

Neutralization Solution B

Synonym(s):

DNA neutralization solution, neutralization buffer DNA extraction

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24 ML
CN¥993.93

CN¥993.93

Available to ship onApril 14, 2025Details

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24 ML
CN¥993.93

About This Item

UNSPSC Code:
12161703
NACRES:
NA.25

CN¥993.93

Available to ship onApril 14, 2025Details

grade

Molecular Biology

Quality Level

200

form

liquid

technique(s)

DNA amplification: suitable
DNA extraction: suitable

storage temp.

−20°C

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This Item
N9784SRE0087D5688
Neutralization Solution B

N3910

Neutralization Solution B

Neutralization Solution for Blood sufficient for 100 reactions, sufficient for 1000 reactions

N9784

Neutralization Solution for Blood

Sigma-Aldrich

SRE0087

Neutralization Solution for Blood

Dilution Solution Extract-N-Amp Plant Dilution Solution

D5688

Dilution Solution

technique(s)

DNA amplification: suitable, DNA extraction: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

General description

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Application

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure.[1] It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.[2]

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000318607
    0000381622
    0000381620
    0000315468
    0000318604

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    Yan Zhang et al.
    American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
    Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
    Işın Çakır et al.
    Nature metabolism, 4(1), 44-59 (2022-01-19)
    The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
    Jun He et al.
    Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
    Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
    A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
    Varshney GK, et al.
    Nature Protocols, 11(12), 2357-2357 (2016)
    Yan Zhang et al.
    American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
    Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
    View All Related Papers

    Protocols

    SYBR® Green Extract-N-Amp™ PCR Kit

    SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service