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Merck
CN

P7059

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonym(s):

Phosphate buffered saline, PBS

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:
MFCD00131855

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Product Name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

Quality Level

200

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Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Preparation Note

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate
Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000492632
0000492632
0000468486
0000468486
0000450199

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Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.

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