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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonym(s):

Sodium Borate Electrophoresis Buffer

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5 EA
CN¥768.14

CN¥768.14

Estimated to ship onMay 23, 2025Details

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5 EA
CN¥768.14

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

CN¥768.14

Estimated to ship onMay 23, 2025Details

Request a Bulk Order

grade

Molecular Biology

Quality Level

200

description

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

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specific activity

~50 U/mg

specific activity

≥1,500 units/mg protein (biuret)

specific activity

300-1,500 units/mg protein

specific activity

1,000-3,000 units/mg protein

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

color

yellow-brown

color

-

color

-

color

-

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures[1]
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Caution

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H319,H360FD

Hazard Classifications

Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000371239
    0000371237
    0000371239
    0000371237
    0000279962

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    Protocols

    Northern & Southern Blot Protocols

    Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service