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1.52022

Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

别名:

Chromolith® HighResolution RP-18 封端100-4.6 HPLC柱

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About This Item

UNSPSC代码:
41115709
NACRES:
SB.52

product name

Chromolith® HighResolution RP-18 封端, L × I.D. 100 mm x 4.6 mm HPLC column

物料

PEEK column

质量水平

Agency

suitable for USP L1

产品线

Chromolith®

特点

endcapped
endcapped

包装

1 ea of

标记范围

15% Carbon loading
18% Carbon loading

参数

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

技术

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

色谱柱长度 × 内径

10 cm × 4.6 mm

表面积

250 m2/g × 250 m2/g

基质

highly porous
monolithic silica
monolithic silica

基质活性基团

C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase

孔径

1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity

operating pH range

2-7.5
2-7.5

分离技术

reversed phase

储存温度

no temp limit

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相关类别

一般描述

Chromolith®色谱柱均由具有特征性双峰孔结构的硅胶整体制成

Chromolith®高分辨率RP-18封端柱具有直径1.15 μm的大孔隙,色谱柱效率超过140,000塔板/米。中孔为15 nm(150 Å),表面修饰为带有完整封端的十八烷基硅烷。Chromolith®高分辨率RP-18封端柱属于USP L1类。柱效率可与2.7至3μm粒径全孔隙颗粒柱相媲美。Chromolith®高分辨率RP-18封端柱非常适用于LC-MS应用。
柱长100 mm,内径4.6 mm,是所有HPLC系统的高分辨率分离的不二之选。

这种突破性硅胶整体以其独特的大孔和中孔结构组合实现了以下功能:
—在极低的柱背压下实现快速分离
—具有高容忍度基质,简化工作流程,同时省时省钱
—延长柱使用寿命,实现成本节约
—连接柱、流动梯度及使用粘度较高的移动相
推荐保护柱为:
Chromolith®高分辨率RP-18封端5-4.6保护小柱(3件)[1.52025]和Chromolith®保护小柱支架5 mm x 4.6 mm[1.52032]
。Chromolith®柱耦合器可通过串联多个柱(高达100,000板/柱)来显著提高Chromolith®柱的分离效率。[1.51467]

了解更多:
宣传册:在任何系统上快速完成您的分离操作。Chromolith®硅胶整体HPLC柱

所有Supelco HPLC柱,包括Chromolith®整体柱,与所有HPLC和UHPLC仪器完全兼容。
Supelco HPLC产品:所有LC仪器的最佳选择

Chromolith®高分辨率RP-18封端柱也适用于2 mm内径柱,满足UHPLC和UHPLC-MS的需要。
传单:与UHPLC性能类似 - 低压下快速且稳定 - Chromolith®高分辨率RP-18封端2 mm内径柱

应用


  • A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).

分析说明

外观:通过测试
压力:40-70 bar
稳定性测试:通过测试
性能(蒽)
理论塔板数(N/m)(蒽): ≥ 140000
容量系数(蒽):3.9 - 4.6
拖尾因子(USP):0.90 - 1.30 %
选择性
分离因子(蒽/孕酮):1.95 - 2.35

法律信息

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids


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商品

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

实验方案

Folic acid vs. folate differences and biological activities; implications in fortified foods and supplements for human health.

相关内容

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

小分子特指低分子量化合物(通常小于900道尔顿),分离小分子方法包括高效液相色谱(HPLC)、液相(LC)、气相(GC)、薄层色谱(TLC) 和毛细管电泳(CE)。此外,还可通过磁共振波谱法(NMR)或 质谱法(MS)等方法鉴定小分子。

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

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