跳转至内容
Merck
CN

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles application for HPLC

材料

标准

产品编号
说明
价格

L4631

Lysozyme 来源于鸡蛋白

For use as a marker in SDS-PAGE

L6876

Lysozyme 来源于鸡蛋白

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

CONDITIONS

column

SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles (Z777051)

mobile phase

50 mM phosphate buffered saline, 200 mM Na2SO4, pH 7.0

flow rate

1 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL in 50 mM phosphate buffered saline

说明

分析说明

The positively-charged protein, lyzoyme (pI=11), exhibits excellent peak shape and recovery on SRT SEC-300 columns due to its highly-inert surface. Low protein recovery is usually attributed to 1) hydrophobic interactions with the stationary phase and 2) electrostatic interactions with the silica suface. SRT SEC-300 surface is hydrophilic and densely bonded to silica, therefore providing high mass recovery and good peak shape.

法律信息

SRT is a registered trademark of Sepax Technologies

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS