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Merck
CN

L4631

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

For use as a marker in SDS-PAGE

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

chicken egg white

质量水平

形式

powder

分子量

single-chain 14.3 kDa

包装

vial of 25 mg

UniProt登记号

储存温度

2-8°C

基因信息

chicken ... LYZ(396218)

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应用

酶可分解细菌的细胞壁;用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。

单位定义

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

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Enav Bar Shira et al.
PloS one, 13(7), e0200393-e0200393 (2018-07-07)
Intestinal epithelial cells are multi-tasked cells that participate in digestion and absorption as well as in protection of the digestive tract. While information on the physiology and immune functions of intestinal epithelial cells in mammals is abundant, little is known
A Van Haga et al.
Journal of economic entomology, 105(6), 1878-1889 (2013-01-30)
Chalkbrood, caused by Ascosphaera apis (Maassen and Claussen) Olive and Spiltor, is a cosmopolitan fungal disease of honey bee larvae (Apis mellifera L.) for which there is no chemotherapeutic control. We evaluated the efficacy of lysozyme-HCl, an inexpensive food-grade antimicrobial
Tao G Dong et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(7), 2623-2628 (2013-01-31)
Type VI protein secretion system (T6SS) is important for bacterial competition through contact-dependent killing of competitors. T6SS delivers effectors to neighboring cells and corresponding antagonistic proteins confer immunity against effectors that are delivered by sister cells. Although T6SS has been
Thomas Gibaud et al.
Physical review letters, 110(5), 058303-058303 (2013-02-19)
We show that gels formed by arrested spinodal decomposition of protein solutions exhibit elastic properties in two distinct frequency domains, both elastic moduli exhibiting a remarkably strong dependence on volume fraction. Considering the large difference between the protein size and
Andrea Weeks et al.
Eye & contact lens, 39(2), 179-185 (2013-02-16)
This study was designed to assess the duration of hyaluronic acid (HA) release from model contact lens materials when HA was physically incorporated into the hydrogel during synthesis and to assess the effects of the HA release on lysozyme sorption.

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