HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX
材料
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说明
价格
血红蛋白 来源于牛血液
from bovine blood, powder, for Neisseria spp., pack of 100 g or 500 g, suitable for microbiologyCONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase
[A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid
gradient
0 to 32% B in 20 min. (linear)
flow rate
0.5 mL/min
column temp.
35 °C
detector
UV, 280 nm
injection
50 μg each Hb variants (A2, S and A0)
说明
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany