HPLC Analysis of Glutamic Acid Enantiomers on Astec® CHIROBIOTIC® T (25 °C)
材料
保护柱套
产品编号
说明
价格
分析色谱柱
产品编号
说明
价格
标准
产品编号
说明
价格
L-谷氨酸
from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%L-谷氨酸
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 205 nm
injection
10 μL
sample
300 μg /mL in 30:70 water:methanol
说明
法律信息
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC