跳转至内容
Merck
CN
  • A reconstituted mammalian APC-kinesin complex selectively transports defined packages of axonal mRNAs.

A reconstituted mammalian APC-kinesin complex selectively transports defined packages of axonal mRNAs.

Science advances (2020-03-24)
Sebastian Baumann, Artem Komissarov, Maria Gili, Verena Ruprecht, Stefan Wieser, Sebastian P Maurer
摘要

Through the asymmetric distribution of messenger RNAs (mRNAs), cells spatially regulate gene expression to create cytoplasmic domains with specialized functions. In neurons, mRNA localization is required for essential processes such as cell polarization, migration, and synaptic plasticity underlying long-term memory formation. The essential components driving cytoplasmic mRNA transport in neurons and mammalian cells are not known. We report the first reconstitution of a mammalian mRNA transport system revealing that the tumor suppressor adenomatous polyposis coli (APC) forms stable complexes with the axonally localized β-actin and β2B-tubulin mRNAs, which are linked to a kinesin-2 via the cargo adaptor KAP3. APC activates kinesin-2, and both proteins are sufficient to drive specific transport of defined mRNA packages. Guanine-rich sequences located in 3'UTRs of axonal mRNAs increase transport efficiency and balance the access of different mRNAs to the transport system. Our findings reveal a minimal set of proteins sufficient to transport mammalian mRNAs.

材料
货号
品牌
产品描述

Sigma-Aldrich
HEPES, ≥99.5% (titration)
Sigma-Aldrich
磷酸钠 二元 七水合物, ACS reagent, 98.0-102.0%
Sigma-Aldrich
氯化钴 六水合物, ACS reagent, 98%
Sigma-Aldrich
磷酸钠 一元, BioReagent, for molecular biology, anhydrous, ≥98%
Sigma-Aldrich
d-脱硫生物素, ≥98% (TLC)
Sigma-Aldrich
DL-二硫苏糖醇, ≥98% (HPLC), ≥99.0% (titration)
Sigma-Aldrich
S-亚硝基-N-乙酰基-DL-青霉胺, ≥97%, powder
Sigma-Aldrich
咪唑, ACS reagent, ≥99% (titration)
Sigma-Aldrich
L-精氨酸, reagent grade, ≥98%
Sigma-Aldrich
氯化钾, BioXtra, ≥99.0%