H3375
HEPES
≥99.5% (titration)
别名:
4-（2-羟乙基）哌嗪-1-乙磺酸, N-（2-羟乙基）哌嗪-N′-（2-乙磺酸）
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产品名称
HEPES, ≥99.5% (titration)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
color
white
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 500 mg/mL, clear, colorless
suitability
suitable for component for culture media
suitable for enzyme extraction
application(s)
diagnostic assay manufacturing
general analytical
life science and biopharma
pharmaceutical
Quality Level
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Application
- 作为Danieau培养基中的一种成分，用于防止斑马鱼胚胎原肠胚形成后的黑色素形成
- 作为Hank溶液中的一个组分用于铁的降解检测
- 作为HEPES培养基中的一个组分用于细胞培养物的质粒转染
- 与RPMI-1640、L-谷氨酰胺、FBS、丙酮酸钠和β-巯基乙醇用于大鼠胰岛瘤细胞的培养
- 作为TCM199培养基中的一种补充剂用于核转移研究所需的新鲜卵母细胞洗涤
Features and Benefits
- 生化和生物学研究的高纯度产品
- 适用于细胞培养基
- 25°C 时，极易溶于水，pH 值范围为 6.8 - 8.2，pKa 为 7.5
General description
HEPES 广泛应用于细胞培养，尽管细胞呼吸产生的二氧化碳浓度波动，但 HEPES 在维持生理 pH 值方面表现出色，这一特征在碳酸氢盐缓冲液中并不突出。它可以作为两性分离剂，在等电聚焦中产生 pH 梯度，与含有较少取代胺的缓冲液（如 Tris）相比，它对 DNA-限制性内切酶反应的干扰更小。除了这些应用之外，HEPES 还可以应用于各种生物和生化研究，包括免疫沉淀、细胞裂解和活细胞成像。通用性使其成为各种研究应用中不可或缺的工具。
Other Notes
Legal Information
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the purpose of using HEPES in cell culture?
HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.
What is the concentration of HEPES used in cell culture?
HEPES is typically used in cell culture in the range of 10-25 mM.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
可生成组织和干细胞来源3D脑、胃肠道、肺和癌症肿瘤类器官模型的类器官培养产品。
相关内容
了解生产规模的高纯度生物学缓冲液通过我们专业制造溶液，确保稳定性、pH控制和稳定供应。
Discover high-purity biological buffers for production scale. Ensure stability, pH control, and consistent supply with our expert manufacturing solutions.
HEPES Specification Comparison
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