LC/MS/MS Analysis of Pesticide Residues in Dates on the Ascentis^® Express RP-Amide Column after QuEChERS Extraction

技术

LC/MS: suitable
solid phase extraction (SPE): suitable

试验参数

sample/matrix: 5 g ground dates spiked with pesticides at 10 ng/g
extraction process: add 10 mL water and 10 mL acetonitrile; shake 1 hr at 2500 rpm; add contents of Supel QuE citrate extraction tube (55227-U); . (shake for 1 minute; centrifuge at 5000 rpm fo 5 minutes and draw off supernatant)
clean-up tube: Supel QuE PSA/C18 (55288-U)
clean-up process: transfer 1 mL of the extract to 2 mLSupel QuE PSA/C18 tube; shake for 1 minute; centrifuge at 5000 rpm for 3 min; draw off supernatant for analysis.
sample preparation: Dilute 100 μL of cleaned extract to a final volume of 1 mL in 5 mM ammonium formate/0.1% formic acid in water; transfer diluted sample to vial for LC/MS/MS analysis
column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2 μm particles (51576-U)
mobile phase: (A) 5 mM ammonium formate with 0.1% formic acid in water; (B) 5 mM ammonium formate with 0.1% formic acid in 95:5 acetonitrile:H2O
gradient: held at 5% B from 0 to 1 min; to 100% B in 13 min; held at 100% B for 1.5 min; to 95% A in 0.5 min; held at 95% A for 1.5 min
flow rate: 0.4 mL/min
column temp.: 30 °C
detector: MS, ESI (+), MRM
injection: 5 μL

适用性

application for LC-MS
application for SPE

应用

food and beverages