HPLC Analysis of Proteins by Size Exclusion on Zenix^® SEC-300 Columns, Effect of Flow Rate (15 cm column)

技术

HPLC: suitable

试验参数

column: Zenix SEC-300, 15 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777030)
mobile phase: 150 mM sodium phosphate, pH 7
flow rate: as indicated on figure
column temp.: ambient
detector: UV, 214 nm
injection: 10 μL
sample: each 1 mg/mL except uracil, 2.5 mg/mL

适用性

application for HPLC

应用

life science and biopharma
pharmaceutical (small molecule)