技术
HPLC: suitable
试验参数
column: Ascentis Express F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U)
mobile phase: [A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)
flow rate: 0.4 mL/min
pressure: 5178 psi (357 bar)
column temp.: 40 °C
detector: ESI(+), 100-1000 m/z
injection: 2 μL, each compound 300 ng/mL in 40:60 (water:methanol)
适用性
application for HPLC
应用
clinical
vitamins, nutraceuticals, and natural products
分析说明
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows that a combination of Ascentis Express F5 with MS detection provided rapid resolution of seven such compounds. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
1α,25-Dihydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
1α,25-Dihydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
25-Hydroxyvitamin D3 solution, 100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
3-epi-25-Hydroxyvitamin D3, 98% (CP)
3-epi-25-Hydroxyvitamin D2 solution, 100 μg/mL in ethanol, 98% (CP)
1α-Hydroxyvitamin D3, ≥97.0% (HPLC)
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