技术
HPLC: suitable
试验参数
column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4 (adjusted with acetic acid); [B] acetonitrile; (80:20, A:B)
gradient: 20 to 100% B in 10 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ESI(-) in SIR mode
injection: 5 μL
sample: 10 μg/mL
适用性
application for HPLC
应用
clinical
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Dehydrocholic acid
Cholic acid
Chenodeoxycholic acid
Deoxycholic acid
Lithocholic acid
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