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53826-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

别名:

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

物料

stainless steel column

质量水平

100

Agency

suitable for USP L1

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

参数

60 °C temp. range
600 bar max. pressure (9000 psi)

技术

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

长度 × 内径

5 cm × 4.6 mm

表面积

135 m2/g

杂质

<5 ppm metals

基质

Fused-Core particle platform
superficially porous particle

基质活性基团

C18 (octadecyl) phase

粒径

2.7 μm

孔径

90 Å

工作pH值

2-9

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

Ascentis Express HPLC色谱柱通过熔融核® 颗粒技术，可为您提供亚2 μm颗粒的高速度和高效率，同时保持较低的背压。高效率和低背压的结合使UPLC® （或其他超高压系统）用户及传统HPLC用户受益。
请见 Ascentis Express 主页，了解有关这项新色谱柱技术的更多信息。

应用

在Ascentis® Express C18，2.7 μm上对辣椒粉中的辣椒素进行HPLC分析
在Ascentis® Express C30上对比Ascentis® Express C18对玉米油进行UHPLC/MS分析
在Ascentis® Express C18，2.7 μm上对扑热息痛和杂质进行UHPLC分析
在Ascentis® Express C18，2.7 μm上对对羟基苯甲酸酯进行HPLC分析

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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GHS07

警示用语：

Warning

危险声明

H317

预防措施声明

P280

危险分类

Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
USUT007294
USUT007293
USUT007292
USUT007291
USUT007290

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is special about Ascentis Express?

    Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  4. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

  5. Can I use Ascentis Express on any type of HPLC system?

    Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

  6. Can I use Ascentis Express on a UHPLC system?

    Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

  10. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

  11. What flow rate should I use with Ascentis® Express HPLC Columns?

    Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

  12. Can Ascentis® Express HPLC Columns be used for LC-MS?

    Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

  13. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation
Arena K, et al.
Molecules (Basel), 27(5), 1727-1727 (2022)
Katia Arena et al.
Analytical and bioanalytical chemistry, 415(13), 2371-2382 (2022-07-15)
In this work, a comprehensive two-dimensional liquid chromatography system, comprised of a ZIC-HILIC and C18 columns in the first and second dimensions, respectively, was tuned and employed for attaining high resolution profiles of the polyphenolic pattern in seven commercial berry
Stefany Grutzmann Arcari et al.
Electrophoresis, 41(20), 1784-1792 (2020-08-12)
This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two-dimensional liquid chromatography (LC × LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid, and acetonitrile) was used
Adriana Arigò et al.
Journal of chromatography. A, 1649, 462183-462183 (2021-05-27)
In the European Union, cosmetic products are regulated by the Cosmetics Regulation (EC) No. 1223/2009. The Analytical Working Group of the International Fragrance Association (IFRA) suggested that a new sensitive analytical method is needed to determine psoralens in finished cosmetic
Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
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