LC/MS Analysis of Amino Acids on Ascentis^® Express HILIC

技术

HPLC: suitable

试验参数

column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 13 mM ammonium acetate in 10:90 (v/v) water: acetonitrile; [B] 13 mM ammonium acetate in water
gradient: held at 0% B for 1 min; 0 to 90% B in 19 min
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ESI(+)
injection: 10 μL
sample: 10 mg/L in Mobile Phase A

适用性

application for HPLC

应用

life science and biopharma